Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181503_xu46 TGGTGTGATGCCTTCTTGTAGTGTTCTCTGGG(+AGGACA)GATCCCTCATACCCCAGCCACAGCACAGTTT NGG 10 8 17 8 24 0 100 5 10
    240613181501_a1w2 TCTGGAAACTGTGCTGTGGCTGGGGTATGAGG(+AGGACA)GATCCCCAGAGAACACTACAAGAAGGCATCA NGG 10 8 17 8 24 0 100 5 10
    240613181500_c3rd TACCCCTCCTGTGTGTGAAGGCTCTGCTGAAG(+AGGACA)GTACCTTTGGGTCTGGTCTGACCTTACTGCT NGG 10 8 17 8 24 0 100 5 10
    240613181457_cqds GACAAAGCAGTAAGGTCAGACCAGACCCAAAG(+AGGACA)GTACCTTCAGCAGAGCCTTCACACACAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613181456_8812 CGGGCCACCACCTGCCTCTCTCTTATCCAGTG(+AGGACA)GTACCGCCCAGGCCTTAAATCACCCCGTTCC NGG 10 8 17 8 24 0 100 5 10
    240613181454_l47j CACTGGGAACGGGGTGATTTAAGGCCTGGGCG(+AGGACA)GTACCACTGGATAAGAGAGAGGCAGGTGGTG NGG 10 8 17 8 24 0 100 5 10
    240613181452_vmtq GGTCACAGGGACAGCTCCGCTGCTGCCTCTGG(+AGGACA)GTACCTTGAAAAGATGCCCACCACGCACCTT NGG 10 8 17 8 24 0 100 5 10
    240613181451_1py8 AAAGAAAGGTGCGTGGTGGGCATCTTTTCAAG(+AGGACA)GTACCCAGAGGCAGCAGCGGAGCTGTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240613181448_3atg CTGCTGCCCCTGGGTGCAGGCAGTAGCCAGGG(+AGGACA)GATCCAGGGCCTCAGATCCAGGGTCCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240613181446_2yv1 GTCCGATGCAGGACCCTGGATCTGAGGCCCTG(+AGGACA)GATCCCCTGGCTACTGCCTGCACCCAGGGGC NGG 10 8 17 8 24 0 100 5 10