Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181557_br8a TCTATGGGCATTGGCCCTGACCTAGGAGTCAG(+AGGACA)GATCCCAGGTCACACCTATGGGTGCTGGCTA NGG 10 8 17 8 24 0 100 5 10
    240613181554_nwig GTCACTAGCCAGCACCCATAGGTGTGACCTGG(+AGGACA)GATCCTGACTCCTAGGTCAGGGCCAATGCCC NGG 10 8 17 8 24 0 100 5 10
    240613181553_y69q ACTGAGGTAGGCAGGAGGGCTGGGGTGCTTGG(+AGGACA)GTACCCCATAATCAGCACAGATGAGGTTGGA NGG 10 8 17 8 24 0 100 5 10
    240613181551_yv2i GTCCTTCCAACCTCATCTGTGCTGATTATGGG(+AGGACA)GTACCCAAGCACCCCAGCCCTCCTGCCTACC NGG 10 8 17 8 24 0 100 5 10
    240613181549_w8zb AAGCCCTTTGTACTCCCTGGGAATCCCCACTG(+AGGACA)GATCCCAGAGACATAGCTTAGCTTTGTCTTC NGG 10 8 17 8 24 0 100 5 10
    240613181547_8k7o ACCAAGAAGACAAAGCTAAGCTATGTCTCTGG(+AGGACA)GATCCAGTGGGGATTCCCAGGGAGTACAAAG NGG 10 8 17 8 24 0 100 5 10
    240613181545_xtp5 GTCCTTAGTCTACAGTGCCTGCTCTTTTACCG(+AGGACA)GTACCACCTCACCTCACTTCAGGAGGGCAAG NGG 10 8 17 8 24 0 100 5 10
    240613181544_51ks AATGCCTTGCCCTCCTGAAGTGAGGTGAGGTG(+AGGACA)GTACCGGTAAAAGAGCAGGCACTGTAGACTA NGG 10 8 17 8 24 0 100 5 10
    240613181542_9t4w CGAGTGTATGTGAATGTTTTGGCGGTGGCCAG(+AGGACA)GATCCCAGGCAAGGGATGGTGTGGGGACTGA NGG 10 8 17 8 24 0 100 5 10
    240613181540_9445 GGGTGTCAGTCCCCACACCATCCCTTGCCTGG(+AGGACA)GATCCTGGCCACCGCCAAAACATTCACATAC NGG 10 8 17 8 24 0 100 5 10