Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181444_woo4 CTGCCGGGGAGATGGGCAGGGTGCACTCTGAG(+AGGACA)GTACCCGCTCCTACAGTCCCAGAACACCCCT NGG 10 8 17 8 24 0 100 5 10
    240613181442_samy AAGAGAGGGGTGTTCTGGGACTGTAGGAGCGG(+AGGACA)GTACCTCAGAGTGCACCCTGCCCATCTCCCC NGG 10 8 17 8 24 0 100 5 10
    240613181441_vp46 CCAGCCACGCACAAGGGGTGGGAGTGGAGTGG(+AGGACA)GATCCTTAGACTGAAATGCGGTGACACATGG NGG 10 8 17 8 24 0 100 5 10
    240613181439_omwd GAGGACCATGTGTCACCGCATTTCAGTCTAAG(+AGGACA)GATCCCACTCCACTCCCACCCCTTGTGCGTG NGG 10 8 17 8 24 0 100 5 10
    240613181438_sbkv GCCTTTGCAGAGAAATGTTAATCCTGCGTGAG(+AGGACA)GTACCAGCTGCTCCCAGGACCCTCCGAGCGA NGG 10 8 17 8 24 0 100 5 10
    240613181436_0crq TACTCTCGCTCGGAGGGTCCTGGGAGCAGCTG(+AGGACA)GTACCTCACGCAGGATTAACATTTCTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240613181434_120h CACATCTCAGAAACAATATCTTGTTGCCAATG(+AGGACA)GTACCCACACATTACATGTCCATGCATACAC NGG 10 8 17 8 24 0 100 5 10
    240613181433_tgtl GCATGGTGTATGCATGGACATGTAATGTGTGG(+AGGACA)GTACCATTGGCAACAAGATATTGTTTCTGAG NGG 10 8 17 8 24 0 100 5 10
    240613181431_7jzz GCTTACTTTCTACAAGCTAACACGGTCTACTG(+AGGACA)GATCCCGCTTATTTCAAGGTGGGCTTTCATT NGG 10 8 17 8 24 0 100 5 10
    240613181430_pj0k TCACCAATGAAAGCCCACCTTGAAATAAGCGG(+AGGACA)GATCCAGTAGACCGTGTTAGCTTGTAGAAAG NGG 10 8 17 8 24 0 100 5 10