Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181730_p9xr TCTCCAAGAGATATTTGCATATACATTAACAG(+AGGACA)GTACCAGTAACCCGTGTGTGTCTCCAATTAA NGG 10 8 17 8 24 0 100 5 10
    240613181729_wh0f ATCCATTAATTGGAGACACACACGGGTTACTG(+AGGACA)GTACCTGTTAATGTATATGCAAATATCTCTT NGG 10 8 17 8 24 0 100 5 10
    240613181728_p31x TCTCTCCTCATTCCTACTCTGAGGTCTCTCAG(+AGGACA)GATCCCGGTTGCCCCGCACCTCCACTTGTGC NGG 10 8 17 8 24 0 100 5 10
    240613181726_0335 GGCAGGCACAAGTGGAGGTGCGGGGCAACCGG(+AGGACA)GATCCTGAGAGACCTCAGAGTAGGAATGAGG NGG 10 8 17 8 24 0 100 5 10
    240613181724_n138 GCTTATCCTCAATCACAAAGAAAAGAAGCTGG(+AGGACA)GTACCAAAATTCTTTATTTGAAGAAATGGTA NGG 10 8 17 8 24 0 100 5 10
    240613181723_d96p ATTTGTACCATTTCTTCAAATAAAGAATTTTG(+AGGACA)GTACCCAGCTTCTTTTCTTTGTGATTGAGGA NGG 10 8 17 8 24 0 100 5 10
    240613181721_b2dx GATGTGTCTGCTCAGCTTCTTAGTCTAGCAGG(+AGGACA)GATCCCCTTCTCTCTGGGAAGAAACTGATTA NGG 10 8 17 8 24 0 100 5 10
    240613181719_n58n AAAGGTAATCAGTTTCTTCCCAGAGAGAAGGG(+AGGACA)GATCCCTGCTAGACTAAGAAGCTGAGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240613181718_c0iv ACAACGTCTGTGTTAGCGTTCTTGGGAAAGAG(+AGGACA)GATCCTTAATCGATACACTTGCCATATCCAG NGG 10 8 17 8 24 0 100 5 10
    240613181716_u9go GTTCCCTGGATATGGCAAGTGTATCGATTAAG(+AGGACA)GATCCTCTTTCCCAAGAACGCTAACACAGAC NGG 10 8 17 8 24 0 100 5 10