Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613181804_pgg7 GCCAGCCAGTAATGACCACATCTCTCCATAGG(+AGGACA)GATCCATGGGGAAGCTGGTGTTCGTCGGATA NGG 10 8 17 8 24 0 100 5 10
    240613181801_nl8o ACCTTTATCCGACGAACACCAGCTTCCCCATG(+AGGACA)GATCCCTATGGAGAGATGTGGTCATTACTGG NGG 10 8 17 8 24 0 100 5 10
    240613181800_xjg8 AGGACTTTCAGATGGATTGATGAATCTTGCTG(+AGGACA)GTACCAGTTTCACTACCAAAAGGTTGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240613181758_lev0 GCTCCTTTGACAACCTTTTGGTAGTGAAACTG(+AGGACA)GTACCAGCAAGATTCATCAATCCATCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240613181757_6ncm ACCCAGTCACTTTCCTACAATCCCACATAGAG(+AGGACA)GATCCATTTTCTCTGGGAAGGAAGTTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240613181755_g8rk ATACTGAGAAAACTTCCTTCCCAGAGAAAATG(+AGGACA)GATCCTCTATGTGGGATTGTAGGAAAGTGAC NGG 10 8 17 8 24 0 100 5 10
    240613181754_crgr GAGTATCTCTTTGAAAAGCCTCATTAAGCTGG(+AGGACA)GTACCACTGCCTGTCATGGTGGTCAGTATAT NGG 10 8 17 8 24 0 100 5 10
    240613181752_rlqu TATCCATATACTGACCACCATGACAGGCAGTG(+AGGACA)GTACCCAGCTTAATGAGGCTTTTCAAAGAGA NGG 10 8 17 8 24 0 100 5 10
    240613181751_3d1a GTAGGAGGCCACTAGCTACTTCCCAAAGTGAG(+AGGACA)GATCCACCCAGAATCCCCCAGGCCCCACTGT NGG 10 8 17 8 24 0 100 5 10
    240613181749_14rj GAGCTACAGTGGGGCCTGGGGGATTCTGGGTG(+AGGACA)GATCCTCACTTTGGGAAGTAGCTAGTGGCCT NGG 10 8 17 8 24 0 100 5 10