Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174343_0j7r TAGAACTACCTTAAGACAGGCAGGAGGCTTGG(+AAAAAA)GTACCTCCTATTCTCCAACATCCTTGCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606174341_p70n GAAAAATAAATTAAGAAATCAAAACAACACTG(+AAAAAA)GATCCATAAGCAATCCGAAGCCGCTTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240606174340_ik9t AGCGACAAGCAAGCGGCTTCGGATTGCTTATG(+AAAAAA)GATCCAGTGTTGTTTTGATTTCTTAATTTAT NGG 10 8 17 8 24 0 100 5 10
    240606174339_kqh4 AAGACAGATAGAAACCTGATTTCTCTCACTGG(+AAAAAA)GATCCACAAGGTTAAAGGAGGAAAAAAATGA NGG 10 8 17 8 24 0 100 5 10
    240606174337_cnv0 GCAAGTCATTTTTTTCCTCCTTTAACCTTGTG(+AAAAAA)GATCCCAGTGAGAGAAATCAGGTTTCTATCT NGG 10 8 17 8 24 0 100 5 10
    240606174336_r8sc GACAAGAAAAACAACTGTGATGTGGGCAGCAG(+AAAAAA)GTACCTGGGGGTGGAGGGTCTTATTGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606174333_gojv CCTGGAGAGGCAATAAGACCCTCCACCCCCAG(+AAAAAA)GTACCTGCTGCCCACATCACAGTTGTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240606174332_fib9 CAGGCCAATTATCCTCGGGAAGGAGGGTAAAG(+AAAAAA)GATCCCTCACTATCTTTTGAATTTGGACTGA NGG 10 8 17 8 24 0 100 5 10
    240606174330_uren CTAGATCAGTCCAAATTCAAAAGATAGTGAGG(+AAAAAA)GATCCTTTACCCTCCTTCCCGAGGATAATTG NGG 10 8 17 8 24 0 100 5 10
    240606174329_mnlm GAAGCAGGAGAGGAGAGCAAGGAAGTTGACAG(+AAAAAA)GTACCTTTAACTGTCCACTTGACACAGCCTA NGG 10 8 17 8 24 0 100 5 10