Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174359_z55i ATATTAACAAACAATAACCAAAATATTGAAAG(+AAAAAA)GTACCATTCACAGAACCATTCCTCAAGTGTA NGG 10 8 17 8 24 0 100 5 10
    240606174357_pxmq CTTACCATCACAGTAAGACATAAGTGGGTGAG(+AAAAAA)GATCCCTTCTGTCATTGATTCTTTCTGTAAA NGG 10 8 17 8 24 0 100 5 10
    240606174355_g5wx CACAGTTTACAGAAAGAATCAATGACAGAAGG(+AAAAAA)GATCCTCACCCACTTATGTCTTACTGTGATG NGG 10 8 17 8 24 0 100 5 10
    240606174354_eya5 TCAAAGGCTCAATGCTGCCCTAAATTAAACAG(+AAAAAA)GTACCATGGGCAGTCTAAGTGGGTATTGTGC NGG 10 8 17 8 24 0 100 5 10
    240606174351_7jtj CTCAGGCACAATACCCACTTAGACTGCCCATG(+AAAAAA)GTACCTGTTTAATTTAGGGCAGCATTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240606174350_mu71 TGTGGGAGCAGGTTTCCTGTAGAGCGTTCCAG(+AAAAAA)GTACCACAAGATACAGGGCTCCTATTCAACC NGG 10 8 17 8 24 0 100 5 10
    240606174348_y0x3 TTGGGGGTTGAATAGGAGCCCTGTATCTTGTG(+AAAAAA)GTACCTGGAACGCTCTACAGGAAACCTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606174347_rtib AGTTTGACTATAAGAATTTCACTTCTCAACAG(+AAAAAA)GTACCTGAACAGTACAATATTAACGATATTG NGG 10 8 17 8 24 0 100 5 10
    240606174346_2fjv AAGTACAATATCGTTAATATTGTACTGTTCAG(+AAAAAA)GTACCTGTTGAGAAGTGAAATTCTTATAGTC NGG 10 8 17 8 24 0 100 5 10
    240606174344_8qtt TTGTGGAAGGCAAGGATGTTGGAGAATAGGAG(+AAAAAA)GTACCCAAGCCTCCTGCCTGTCTTAAGGTAG NGG 10 8 17 8 24 0 100 5 10