Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174500_2g5p CTGAGCGCCTCACTGGGAGATTCTAGGCAGGG(+AAAAAA)GATCCACCACAACCATTCCCTAACTCCTCAC NGG 10 8 17 8 24 0 100 5 10
    240606174459_laxb GGGGATGCTGCGGACAAGGCATCCAGGATGTG(+AAAAAA)GATCCCCAAGGAGCCTTCAGAAAAGATGCCC NGG 10 8 17 8 24 0 100 5 10
    240606174457_uh55 GGAGAGGGCATCTTTTCTGAAGGCTCCTTGGG(+AAAAAA)GATCCACATCCTGGATGCCTTGTCCGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240606174454_7sj0 TGCAATTATGGGGATCTGAGTTTGAATCCCTG(+AAAAAA)GTACCCATATAAAGACACAGATGCGGTGATG NGG 10 8 17 8 24 0 100 5 10
    240606174452_pur0 GTGCCCATCACCGCATCTGTGTCTTTATATGG(+AAAAAA)GTACCAGGGATTCAAACTCAGATCCCCATAA NGG 10 8 17 8 24 0 100 5 10
    240606174451_5oza ATCCCCGATCCTAGCCCTGTGTCCTGACCATG(+AAAAAA)GTACCACTGCACCCCACACTTGAGCCACAGG NGG 10 8 17 8 24 0 100 5 10
    240606174450_zq12 GGGATCCTGTGGCTCAAGTGTGGGGTGCAGTG(+AAAAAA)GTACCATGGTCAGGACACAGGGCTAGGATCG NGG 10 8 17 8 24 0 100 5 10
    240606174447_9yts CTTCAAGATTGGATTCTGAGCTTAGACCTGGG(+AAAAAA)GTACCCCGCTCCACCTCTCAGTCTTTAGGGG NGG 10 8 17 8 24 0 100 5 10
    240606174445_lsd4 AGGTCCCCCTAAAGACTGAGAGGTGGAGCGGG(+AAAAAA)GTACCCCAGGTCTAAGCTCAGAATCCAATCT NGG 10 8 17 8 24 0 100 5 10
    240606174443_0gbt TAATCCTAGCACTCAGGAGGTGAGGCAGGTGG(+AAAAAA)GTACCTGAGTCTGAGGCTAGATTGGTTTAGT NGG 10 8 17 8 24 0 100 5 10