Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174531_23n8 GAGTGTCTAAAATGTCTGAAGGGTGACAGATG(+AAAAAA)GATCCATGAATGAGTGCAGCCATTACATTTT NGG 10 8 17 8 24 0 100 5 10
    240606174530_d680 ACATGTAAATTAATATTTAGAGTGAAGGAATG(+AAAAAA)GATCCAATTGATGAAGGAAGATGAGGAAGCC NGG 10 8 17 8 24 0 100 5 10
    240606174528_wq00 TGATAGGCTTCCTCATCTTCCTTCATCAATTG(+AAAAAA)GATCCATTCCTTCACTCTAAATATTAATTTA NGG 10 8 17 8 24 0 100 5 10
    240606174527_ljpe ACCGTTTCGCTAATAGTTACTGCCAGCAGCTG(+AAAAAA)GATCCTGCATACAAAACACTTGTTCATGTTT NGG 10 8 17 8 24 0 100 5 10
    240606174526_6vds AAGGAAAACATGAACAAGTGTTTTGTATGCAG(+AAAAAA)GATCCAGCTGCTGGCAGTAACTATTAGCGAA NGG 10 8 17 8 24 0 100 5 10
    240606174525_a9r7 GTCTGCAGTGGGCAGGGTGCTGGAGGAGAAGG(+AAAAAA)GATCCAGGCAGACCCCACTCCAACTGAACCA NGG 10 8 17 8 24 0 100 5 10
    240606174523_p594 GTATGTGGTTCAGTTGGAGTGGGGTCTGCCTG(+AAAAAA)GATCCCTTCTCCTCCAGCACCCTGCCCACTG NGG 10 8 17 8 24 0 100 5 10
    240606174521_lraw GCATTTTTTTTCCATAGGTAACTAACACCATG(+AAAAAA)GATCCAACCAGGCAGAAGTTTGGAGCAAGTT NGG 10 8 17 8 24 0 100 5 10
    240606174519_oza6 AGCAAAACTTGCTCCAAACTTCTGCCTGGTTG(+AAAAAA)GATCCATGGTGTTAGTTACCTATGGAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240606174517_cw49 AGACCCTATACAAGCAGCATCGCTGTTGCCAG(+AAAAAA)GATCCTCTTCCTAATGAAGGGTCGCAGAGAC NGG 10 8 17 8 24 0 100 5 10