Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174631_1l92 AAACTGGATCAGACTGGTTTTCCCAAGAAATG(+AAAAAA)GATCCAGGGAGAGTGTGGGAAGGGGATGGAA NGG 10 8 17 8 24 0 100 5 10
    240606174630_u772 ATAAAGAAATGTAAACAGTTTAACACCGACAG(+AAAAAA)GATCCCTCTGCTAGTCCTTATTGTGACTGTT NGG 10 8 17 8 24 0 100 5 10
    240606174628_yu4i TCTGGAACAGTCACAATAAGGACTAGCAGAGG(+AAAAAA)GATCCTGTCGGTGTTAAACTGTTTACATTTC NGG 10 8 17 8 24 0 100 5 10
    240606174627_hvkk TGGTGGAGGAAGTTCAGACCACACCTGAACTG(+AAAAAA)GTACCTGAGATGACAGCACTGAATCTGAAGA NGG 10 8 17 8 24 0 100 5 10
    240606174626_2daa CTTTGTCTTCAGATTCAGTGCTGTCATCTCAG(+AAAAAA)GTACCAGTTCAGGTGTGGTCTGAACTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240606174624_0lkq CCAGTGTCTGAGGCCGCCAGGGAGGTGCGGGG(+AAAAAA)GTACCCCACATCCCCACGAGGCCTCATCTGC NGG 10 8 17 8 24 0 100 5 10
    240606174622_msnw ATAGTGCAGATGAGGCCTCGTGGGGATGTGGG(+AAAAAA)GTACCCCCGCACCTCCCTGGCGGCCTCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606174621_6pbl TTGTACAGCTGCGAGGGAGGGCACAGATGCAG(+AAAAAA)GTACCTGCGCACTCCGCCCTTCTCTCTTACA NGG 10 8 17 8 24 0 100 5 10
    240606174620_9fx1 GGGTGTGTAAGAGAGAAGGGCGGAGTGCGCAG(+AAAAAA)GTACCTGCATCTGTGCCCTCCCTCGCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240606174617_y3y2 TTCTAGGCACACTCCTTGCAGTAGGCTCCAGG(+AAAAAA)GTACCTTGGAACTGCAACCAAGGCATGGTAA NGG 10 8 17 8 24 0 100 5 10