Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174721_kyzw ACCAGACAGAGGATGGCAAACACTGTCTTACG(+AAAAAA)GATCCTTGGGACCATCCCTCGCCTTGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240606174719_j2vo ACAAATCACAGCTCTGTGGTGTTAGGGCCGTG(+AAAAAA)GATCCTAAGCTGTGTGAACACAGAGAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606174718_3vu1 CTTTCCCTCCTCTCTGTGTTCACACAGCTTAG(+AAAAAA)GATCCACGGCCCTAACACCACAGAGCTGTGA NGG 10 8 17 8 24 0 100 5 10
    240606174716_9bq9 TTTTACATGGGATACACACGTTAGCCCACCTG(+AAAAAA)GTACCCACCTGGAATATTTCCTAACTGGTTA NGG 10 8 17 8 24 0 100 5 10
    240606174714_9maw TCAAATAACCAGTTAGGAAATATTCCAGGTGG(+AAAAAA)GTACCAGGTGGGCTAACGTGTGTATCCCATG NGG 10 8 17 8 24 0 100 5 10
    240606174712_hl6j TATGAAGCTCTTCCACTTCCATCCAGGTAAGG(+AAAAAA)GATCCAATTGACAGGCAATCTGGTAATACAT NGG 10 8 17 8 24 0 100 5 10
    240606174711_aofd TTTCAATGTATTACCAGATTGCCTGTCAATTG(+AAAAAA)GATCCCTTACCTGGATGGAAGTGGAAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240606174710_pwr8 CCAGGGGATCCAACATTTTCTGATCTCTATGG(+AAAAAA)GTACCACGCAGGCAAAACACTCATATACATT NGG 10 8 17 8 24 0 100 5 10
    240606174708_avdj TATTTAATGTATATGAGTGTTTTGCCTGCGTG(+AAAAAA)GTACCCATAGAGATCAGAAAATGTTGGATCC NGG 10 8 17 8 24 0 100 5 10
    240606174706_k9nd GCTCACAACCATCTGTAACTCCAGTTCCAGGG(+AAAAAA)GATCCAACATTTTCTGATCTCTATGGGTACC NGG 10 8 17 8 24 0 100 5 10