Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174704_r4ix TGCGTGGTACCCATAGAGATCAGAAAATGTTG(+AAAAAA)GATCCCCTGGAACTGGAGTTACAGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240606174703_0t02 TTAGCTCCTAATATGTCCCTTCTCTCACACTG(+AAAAAA)GTACCTCAATTTATGAGTTAAAACTGGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606174702_7zf0 TTTAGAGAGCCAGTTTTAACTCATAAATTGAG(+AAAAAA)GTACCAGTGTGAGAGAAGGGACATATTAGGA NGG 10 8 17 8 24 0 100 5 10
    240606174659_4yh4 GCAGATGGCGTTAAGTGATGTTACTGTCCTGG(+AAAAAA)GTACCTAGAGCCTTTAGTTCATGACACGCTG NGG 10 8 17 8 24 0 100 5 10
    240606174658_28m0 GCAAGCAGCGTGTCATGAACTAAAGGCTCTAG(+AAAAAA)GTACCCAGGACAGTAACATCACTTAACGCCA NGG 10 8 17 8 24 0 100 5 10
    240606174657_1xvx TACCTTAGATCGCACAAAAATGCATAATCATG(+AAAAAA)GATCCCCTACTCTCCCAGTAGTCAAGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240606174655_ya4d TTCCTAAGAACTTGACTACTGGGAGAGTAGGG(+AAAAAA)GATCCATGATTATGCATTTTTGTGCGATCTA NGG 10 8 17 8 24 0 100 5 10
    240606174653_lf91 TCCATGTGGCAACTCAGACCTGCCTAAAACTG(+AAAAAA)GATCCCCTCTTCTGACCTTCATAGTCATCAC NGG 10 8 17 8 24 0 100 5 10
    240606174651_nmst ATTGTGTGATGACTATGAAGGTCAGAAGAGGG(+AAAAAA)GATCCAGTTTTAGGCAGGTCTGAGTTGCCAC NGG 10 8 17 8 24 0 100 5 10
    240606174650_ola9 TGCGATGCATTACATTAGCCGAGGCACTTCAG(+AAAAAA)GATCCTTCTTATACCCATTCTCTCAACAACT NGG 10 8 17 8 24 0 100 5 10