Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174804_ri3j TTGAGATTATTCCGTAAGAACTAGATACCCAG(+AAAAAA)GTACCACCACATGTGCTTTTATGCTTAGTGT NGG 10 8 17 8 24 0 100 5 10
    240606174803_pfw4 GAAGAAGAATCTTGAAAGGGAGGGTGGTGATG(+AAAAAA)GTACCGATAAATACCTGTGTTTTAAAATCTT NGG 10 8 17 8 24 0 100 5 10
    240606174802_bktf TGTACAAGATTTTAAAACACAGGTATTTATCG(+AAAAAA)GTACCATCACCACCCTCCCTTTCAAGATTCT NGG 10 8 17 8 24 0 100 5 10
    240606174800_01a1 TTTAGAATTCAGAGCTAGGTGGGGCTGATGGG(+AAAAAA)GATCCTCTGATCCCGTCTGCCTTGATGGAAG NGG 10 8 17 8 24 0 100 5 10
    240606174758_kccl GTGCTCTTCCATCAAGGCAGACGGGATCAGAG(+AAAAAA)GATCCCCATCAGCCCCACCTAGCTCTGAATT NGG 10 8 17 8 24 0 100 5 10
    240606174757_6nss AAAAGAATATACCTTCTTCTCAGCACCTCATG(+AAAAAA)GTACCTTCTCCAAAATTGACCATATAATCGG NGG 10 8 17 8 24 0 100 5 10
    240606174756_lkee TGTGACCGATTATATGGTCAATTTTGGAGAAG(+AAAAAA)GTACCATGAGGTGCTGAGAAGAAGGTATATT NGG 10 8 17 8 24 0 100 5 10
    240606174754_9b12 CCTCTTCCAAACGACCCTAGTTCAATTTCCAG(+AAAAAA)GATCCACATGGCAACTCATAGATGTCTGTAA NGG 10 8 17 8 24 0 100 5 10
    240606174753_tdlh TGAAGTTACAGACATCTATGAGTTGCCATGTG(+AAAAAA)GATCCTGGAAATTGAACTAGGGTCGTTTGGA NGG 10 8 17 8 24 0 100 5 10
    240606174752_ogec TCAGGGCAGAGAAACTGAGGAGCCTGGAGAAG(+AAAAAA)GATCCCTCCGGTTCCCATCTGTGCCTGGAGC NGG 10 8 17 8 24 0 100 5 10