Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174908_i90t GTAAACATTACACATGTCATTTTGTGGCACAG(+AAAAAA)GATCCCAAAATAAAATATCAAAGTAATCCCA NGG 10 8 17 8 24 0 100 5 10
    240606174907_nmp7 GAGATACATAAGGCACAACAGCTTTCCTTCAG(+AAAAAA)GATCCTTCTCTCTGTCCTAGAGTCTTCAGAC NGG 10 8 17 8 24 0 100 5 10
    240606174906_xeny CTACCGTCTGAAGACTCTAGGACAGAGAGAAG(+AAAAAA)GATCCTGAAGGAAAGCTGTTGTGCCTTATGT NGG 10 8 17 8 24 0 100 5 10
    240606174904_kpp9 CATCAACTGCAAACGTGAGGGGAGAAAGCATG(+AAAAAA)GTACCCAACCAAATTCCACATTTCAGCAATA NGG 10 8 17 8 24 0 100 5 10
    240606174902_v66t TGAAGTATTGCTGAAATGTGGAATTTGGTTGG(+AAAAAA)GTACCATGCTTTCTCCCCTCACGTTTGCAGT NGG 10 8 17 8 24 0 100 5 10
    240606174901_74zi TTTATAAGGAAAAGACTCAAGGTATAAGCTCG(+AAAAAA)GATCCCTAACCCTGTGCTATGTCATCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606174859_desk GTGAGAGAAAGATGACATAGCACAGGGTTAGG(+AAAAAA)GATCCGAGCTTATACCTTGAGTCTTTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240606174858_6g6b TCAGTCCAGAACTAAAAATCAATGTGAAAATG(+AAAAAA)GATCCTCACCTTGAAAGACAAGTGTGACTTC NGG 10 8 17 8 24 0 100 5 10
    240606174857_e3ps AGAAAGAAGTCACACTTGTCTTTCAAGGTGAG(+AAAAAA)GATCCATTTTCACATTGATTTTTAGTTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606174855_215x AGAAAAAATGTGAATACACTTGCTGAGAATTG(+AAAAAA)GATCCTATATATAAAATATCATACCCACATT NGG 10 8 17 8 24 0 100 5 10