Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174839_zzvg AACACTTGCTTGGGGAAAGGGAGCCCAAACAG(+AAAAAA)GATCCCGAGGGAAAGGCAAAGGGGGCATGAG NGG 10 8 17 8 24 0 100 5 10
    240606174837_xzoa AACTGCCATTCTCTGAAGACAGAGCAACAGGG(+AAAAAA)GTACCCCTGCCCCAGGGCTGCTCTCCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240606174834_cutn GAGGGCCAAGGGAGAGCAGCCCTGGGGCAGGG(+AAAAAA)GTACCCCTGTTGCTCTGTCTTCAGAGAATGG NGG 10 8 17 8 24 0 100 5 10
    240606174833_q5tq CCAGAGAGCTCTGAGAGAGGACAAGGCAGCTG(+AAAAAA)GTACCCTGACCAATGTCCTCAGCCACACACA NGG 10 8 17 8 24 0 100 5 10
    240606174831_my0j GAGGGTGTGTGTGGCTGAGGACATTGGTCAGG(+AAAAAA)GTACCAGCTGCCTTGTCCTCTCTCAGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240606174829_7okm GGGGACCCAATTCTTTCTTCTGGCCTCCAAGG(+AAAAAA)GTACCAGCCATACATACATACATACATACAT NGG 10 8 17 8 24 0 100 5 10
    240606174828_lj0m TATGTATGTATGTATGTATGTATGTATGGCTG(+AAAAAA)GTACCCTTGGAGGCCAGAAGAAAGAATTGGG NGG 10 8 17 8 24 0 100 5 10
    240606174826_meds CCCAACCCCTCTAAACCAGCTCTGTGGCTCAG(+AAAAAA)GTACCTTCAGTGAAGCCCATTGCTCTTGCCG NGG 10 8 17 8 24 0 100 5 10
    240606174825_cyta CTGGGCGGCAAGAGCAATGGGCTTCACTGAAG(+AAAAAA)GTACCTGAGCCACAGAGCTGGTTTAGAGGGG NGG 10 8 17 8 24 0 100 5 10
    240606174824_ie4c GAATGAGATTTTGTATTTTCCCTTGAGAGATG(+AAAAAA)GTACCAAGCAGGCAAGCAACCATGTACCTAC NGG 10 8 17 8 24 0 100 5 10