Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174822_1jdo AACTGGTAGGTACATGGTTGCTTGCCTGCTTG(+AAAAAA)GTACCATCTCTCAAGGGAAAATACAAAATCT NGG 10 8 17 8 24 0 100 5 10
    240606174821_8irb AATTGGGTTTTGTCTTCTTTAAAAACAAATAG(+AAAAAA)GTACCGTAAGCATTTTCTGCTGACGACTGCC NGG 10 8 17 8 24 0 100 5 10
    240606174819_b3mw CTTAAGGCAGTCGTCAGCAGAAAATGCTTACG(+AAAAAA)GTACCTATTTGTTTTTAAAGAAGACAAAACC NGG 10 8 17 8 24 0 100 5 10
    240606174818_q74k TGAATAAGAACCAAACTGAGTATTATAGTGAG(+AAAAAA)GATCCTTGACCACTTAGTAGACCTCAGCAAA NGG 10 8 17 8 24 0 100 5 10
    240606174817_0rf8 AGAATTTTGCTGAGGTCTACTAAGTGGTCAAG(+AAAAAA)GATCCTCACTATAATACTCAGTTTGGTTCTT NGG 10 8 17 8 24 0 100 5 10
    240606174816_g215 ACCTTCGCAAAAACTTAAATCATGGAATGGCG(+AAAAAA)GTACCACGGTAGAACACTGCAGCTGTCGCCA NGG 10 8 17 8 24 0 100 5 10
    240606174814_qmxh AACCCTGGCGACAGCTGCAGTGTTCTACCGTG(+AAAAAA)GTACCGCCATTCCATGATTTAAGTTTTTGCG NGG 10 8 17 8 24 0 100 5 10
    240606174811_4dc0 GAATCGACCACCATACCACAGTAACGCCCCTG(+AAAAAA)GATCCCTGGCTGCCCACCCACTCTAAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240606174808_ibiu CAGGATAGCCTTAGAGTGGGTGGGCAGCCAGG(+AAAAAA)GATCCAGGGGCGTTACTGTGGTATGGTGGTC NGG 10 8 17 8 24 0 100 5 10
    240606174806_sack TACAAACACTAAGCATAAAAGCACATGTGGTG(+AAAAAA)GTACCTGGGTATCTAGTTCTTACGGAATAAT NGG 10 8 17 8 24 0 100 5 10