Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174600_xetd CTGGCTTTGCTGAAGAGCTTCTTCCTTCAAAG(+AAAAAA)GATCCTGAGAGAAAACAGCAACACGGCGGAA NGG 10 8 17 8 24 0 100 5 10
    240606174559_ydos GAATAGTTACAAGATGATTTGTGGTATCATGG(+AAAAAA)GTACCCATTATAAACCATGCTCTTCCCAGTA NGG 10 8 17 8 24 0 100 5 10
    240606174557_6t00 TCAGCTACTGGGAAGAGCATGGTTTATAATGG(+AAAAAA)GTACCCATGATACCACAAATCATCTTGTAAC NGG 10 8 17 8 24 0 100 5 10
    240606174555_ighh AGTGAAAAATGATCTTGTTTTTCTTACAGCCG(+AAAAAA)GATCCAAAGACGCTAGATGTTAAGGGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240606174554_nq7e TCAGCCTCAGCCCTTAACATCTAGCGTCTTTG(+AAAAAA)GATCCGGCTGTAAGAAAAACAAGATCATTTT NGG 10 8 17 8 24 0 100 5 10
    240606174552_6v3q GTGGTGGTTGGAAGATCTAAATGGGATTCGGG(+AAAAAA)GTACCCCCAAGCATGCACCCCCCTATTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606174550_19p2 ACTCACTGCAATAGGGGGGTGCATGCTTGGGG(+AAAAAA)GTACCCCGAATCCCATTTAGATCTTCCAACC NGG 10 8 17 8 24 0 100 5 10
    240606174549_kx2f AAGGGTTTCCACTTGGGCTGGACCCAGGTTTG(+AAAAAA)GTACCTAGAAGATCCTTCTGCAGACACCGAG NGG 10 8 17 8 24 0 100 5 10
    240606174547_0r6e AGGTCCTCGGTGTCTGCAGAAGGATCTTCTAG(+AAAAAA)GTACCAAACCTGGGTCCAGCCCAAGTGGAAA NGG 10 8 17 8 24 0 100 5 10
    240606174546_5cet TTCAAATGAGCTGTATAGAAAGACACCTTGTG(+AAAAAA)GTACCTAAAGTGGAACTGCTTCTTCTGTTTC NGG 10 8 17 8 24 0 100 5 10