Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174312_aek7 AACATGGCTTTTTTGGATCTCCTCTGTGTTGG(+AAAAAA)GTACCATATCCTCTTTGCTACCCTTCTATTT NGG 10 8 17 8 24 0 100 5 10
    240606174309_z0d0 GCCAGAAGACAACCTTGGCTCTCATTCCCCAG(+AAAAAA)GTACCATCCAGCTTGGTCTTTAAGATGGGAT NGG 10 8 17 8 24 0 100 5 10
    240606174307_iw0h AAGAGATCCCATCTTAAAGACCAAGCTGGATG(+AAAAAA)GTACCTGGGGAATGAGAGCCAAGGTTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240606174305_a8sj ATAGCCACTATGCCTCAAGATGTCCATACCAG(+AAAAAA)GATCCAGGTCTGAAACTTCTATCTCCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240606174303_4t52 CTGGAGGCTGGGAGATAGAAGTTTCAGACCTG(+AAAAAA)GATCCTGGTATGGACATCTTGAGGCATAGTG NGG 10 8 17 8 24 0 100 5 10
    240606174302_4ng4 TGAGACAACTCAGTTGTCATGTCAGGGGGAAG(+AAAAAA)GATCCTATTTCAGACTGCCTCCTGTGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240606174300_4o5i GGTAGAGGCACACAGGAGGCAGTCTGAAATAG(+AAAAAA)GATCCTTCCCCCTGACATGACAACTGAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606174259_pn6e GAAGTGTCCATGAAGACTGCAACACAGCATTG(+AAAAAA)GATCCCCCTGAGCTGCAGTTACAGACATTTG NGG 10 8 17 8 24 0 100 5 10
    240606174257_4nz5 GCTCACAAATGTCTGTAACTGCAGCTCAGGGG(+AAAAAA)GATCCAATGCTGTGTTGCAGTCTTCATGGAC NGG 10 8 17 8 24 0 100 5 10
    240606174255_03bc CCATAAAGTCTCTGACCATCTCTGGAATCAGG(+AAAAAA)GATCCACCCAGACAGCCTAGACATAGAGCAT NGG 10 8 17 8 24 0 100 5 10