Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174204_udmw CCACCCACCGCCACCATGAGAGGGGTGCCCAG(+AAAAAA)GATCCCCAACAGCCCAGTGCTGCAGGAGTTG NGG 10 8 17 8 24 0 100 5 10
    240606174202_hp67 CTAGGCCAGACATGCCCCTGGGAGTGGAAAAG(+AAAAAA)GATCCCTGTTAGCCCAGCAGCAAGGGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240606174201_3kb4 CCACTTGAGCCCCTTGCTGCTGGGCTAACAGG(+AAAAAA)GATCCTTTTCCACTCCCAGGGGCATGTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606174159_n01h AAGGTTTATGTAAGAGCAGGATTGGGGATGAG(+AAAAAA)GATCCCAGGAGGCACAAACTGGGTCAGGTCC NGG 10 8 17 8 24 0 100 5 10
    240606174157_wjs7 CCATAGGACCTGACCCAGTTTGTGCCTCCTGG(+AAAAAA)GATCCTCATCCCCAATCCTGCTCTTACATAA NGG 10 8 17 8 24 0 100 5 10
    240606174156_ysxr TGAACTACAAGAACGGTTTGCTGTTTCTGCTG(+AAAAAA)GATCCTGTCTTCCTTCCTCAACAAGCCACAA NGG 10 8 17 8 24 0 100 5 10
    240606174155_mzl4 TTTTATTGTGGCTTGTTGAGGAAGGAAGACAG(+AAAAAA)GATCCAGCAGAAACAGCAAACCGTTCTTGTA NGG 10 8 17 8 24 0 100 5 10
    240606174153_kaxs TTGGGGAGCCTCAGGACATGGCATGCTTTGTG(+AAAAAA)GATCCCCATGATAGTCTACAAAAGCACCCCG NGG 10 8 17 8 24 0 100 5 10
    240606174152_ngnz AGGGGCGGGGTGCTTTTGTAGACTATCATGGG(+AAAAAA)GATCCACAAAGCATGCCATGTCCTGAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606174150_x9au GGCCAGGAAAGGGTTAATGAGAGCCAAGGTGG(+AAAAAA)GTACCTGGAACCATGGCTGGAGGCCTGGGGA NGG 10 8 17 8 24 0 100 5 10