Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174132_pf48 TTAGGAAGCCACACTTAGGGATTGGCCCTAAG(+AAAAAA)GTACCAGGAATGATCACAGTACATCCTCCAA NGG 10 8 17 8 24 0 100 5 10
    240606174130_30fm CTTGTTTCACCTCATAAGAACCTCACAAGAAG(+AAAAAA)GTACCAGCAGGTTCTGCCCCAAACTTCACTT NGG 10 8 17 8 24 0 100 5 10
    240606174129_p59j TCCAAAAGTGAAGTTTGGGGCAGAACCTGCTG(+AAAAAA)GTACCTTCTTGTGAGGTTCTTATGAGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240606174128_gma4 GTTCACCCTGCCTGCTTGTAAATTCTAGGAAG(+AAAAAA)GATCCCGGAGAACAGCATGGTTTTCTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240606174126_q222 TTTAATTAAAAGAAAACCATGCTGTTCTCCGG(+AAAAAA)GATCCTTCCTAGAATTTACAAGCAGGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240606174124_8840 ATTCACCATGTCTTATTAATTTCAAAACTAAG(+AAAAAA)GATCCATGCAAGGAGTGGTGTTACAGCCATA NGG 10 8 17 8 24 0 100 5 10
    240606174123_yzyl TTTTTTATGGCTGTAACACCACTCCTTGCATG(+AAAAAA)GATCCTTAGTTTTGAAATTAATAAGACATGG NGG 10 8 17 8 24 0 100 5 10
    240606174122_2yjb GTTGAAGACAGTTTAGCTCCCACAGTGCTGCG(+AAAAAA)GATCCCAGCACAATCCCAGTGCGGTCTCCTT NGG 10 8 17 8 24 0 100 5 10
    240606174120_wj5c AGGGGAAGGAGACCGCACTGGGATTGTGCTGG(+AAAAAA)GATCCGCAGCACTGTGGGAGCTAAACTGTCT NGG 10 8 17 8 24 0 100 5 10
    240606174118_hwzl CTACCTACTAAGCCAGAGAAAGCCGTGGAGGG(+AAAAAA)GTACCCTTAACGTCTTTGATCATTTGCAGGC NGG 10 8 17 8 24 0 100 5 10