Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174043_ozyv GATCTCTGCTTCCTTTCTAGATTCTCAGAGGG(+AAAAAA)GTACCCCCGCCCCTGAAGTGTAGAGGTCAAG NGG 10 8 17 8 24 0 100 5 10
    240606174041_0so1 TTCCCCCAAACACACATCGCAGAAGAAACATG(+AAAAAA)GTACCATTAGCAAAGCTCTGAAACTTTACAG NGG 10 8 17 8 24 0 100 5 10
    240606174040_mmux AGGATCTGTAAAGTTTCAGAGCTTTGCTAATG(+AAAAAA)GTACCATGTTTCTTCTGCGATGTGTGTTTGG NGG 10 8 17 8 24 0 100 5 10
    240606174038_79hx AAGGTAGGCAACAGAGTGGTTTTGGGCACGGG(+AAAAAA)GTACCTCAACTTCGAGGACAGCAAAAACAGC NGG 10 8 17 8 24 0 100 5 10
    240606174037_kxtq CTGAAGCTGTTTTTGCTGTCCTCGAAGTTGAG(+AAAAAA)GTACCCCGTGCCCAAAACCACTCTGTTGCCT NGG 10 8 17 8 24 0 100 5 10
    240606174035_4j5n AATAGGGCCCAGAGGAAAAAAAAATGTGTGAG(+AAAAAA)GATCCACAATGGGTACTTGTGTTTCCTACTG NGG 10 8 17 8 24 0 100 5 10
    240606174034_hwb3 CCTTGCAGTAGGAAACACAAGTACCCATTGTG(+AAAAAA)GATCCTCACACATTTTTTTTTCCTCTGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606174032_7adc GCTTGCTGAGCCCCTTGCAATTAGGAAGACAG(+AAAAAA)GTACCCATTGTGGGTACTCACACATTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240606174031_hrhe CCAGAGAGAAAATGTGTGAGTACCCACAATGG(+AAAAAA)GTACCTGTCTTCCTAATTGCAAGGGGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240606174029_b815 CTCCCTAGAGATCTCAAGGCTAAGGTATAAAG(+AAAAAA)GTACCCTGCCTCATTAGTGGAAAGATGTATA NGG 10 8 17 8 24 0 100 5 10