Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174116_tlxo TCCCTGCCTGCAAATGATCAAAGACGTTAAGG(+AAAAAA)GTACCCCTCCACGGCTTTCTCTGGCTTAGTA NGG 10 8 17 8 24 0 100 5 10
    240606174115_zeqv TAGAGTAGACCATAATATCCCACACTGACGAG(+AAAAAA)GTACCCAAGCAGACCCCTTACATCGGCTTCA NGG 10 8 17 8 24 0 100 5 10
    240606174113_hmf8 TGCTGTGAAGCCGATGTAAGGGGTCTGCTTGG(+AAAAAA)GTACCTCGTCAGTGTGGGATATTATGGTCTA NGG 10 8 17 8 24 0 100 5 10
    240606174112_msbd ACAGGGAAGGAAGCCTGACCCTGAAATGTGAG(+AAAAAA)GATCCCCCCCCCACTTGCAGTCTGCACTTTT NGG 10 8 17 8 24 0 100 5 10
    240606174109_t903 AAAAGAAAAGTGCAGACTGCAAGTGGGGGGGG(+AAAAAA)GATCCTCACATTTCAGGGTCAGGCTTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240606174107_a37v CTTTTATTGAAGCAATAGTAGGTCTGGCTGAG(+AAAAAA)GATCCATCCATAAATACCCTCCACTATTTCT NGG 10 8 17 8 24 0 100 5 10
    240606174106_7qyf TTACAAGAAATAGTGGAGGGTATTTATGGATG(+AAAAAA)GATCCTCAGCCAGACCTACTATTGCTTCAAT NGG 10 8 17 8 24 0 100 5 10
    240606174105_31b6 AGGCAAGAGCATGGTTGTTCGCTTAACAGGAG(+AAAAAA)GATCCTAACACTGTGCAGTGCAAAGAAAGGC NGG 10 8 17 8 24 0 100 5 10
    240606174103_ea2h TAGCAGCCTTTCTTTGCACTGCACAGTGTTAG(+AAAAAA)GATCCTCCTGTTAAGCGAACAACCATGCTCT NGG 10 8 17 8 24 0 100 5 10
    240606174101_qx7o CTGTCTCAGAAGAGCATCTGAACTTGTCCTCG(+AAAAAA)GTACCAAGGAATCTCTCCAACAACTGGGTCT NGG 10 8 17 8 24 0 100 5 10