Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174010_m1ne GAGCTCTGCCTGAGCTCAGTGCTTTACCCCTG(+AAAAAA)GATCCTGGCAGTGTCCTCATCATGGAATGAA NGG 10 8 17 8 24 0 100 5 10
    240606174009_yxfd CTCCACACAAACTGAGACATATATAAGAAGAG(+AAAAAA)GATCCTGAATTGAGAAATTGCTTCCATCCAG NGG 10 8 17 8 24 0 100 5 10
    240606174007_0ldm GCGTCCTGGATGGAAGCAATTTCTCAATTCAG(+AAAAAA)GATCCTCTTCTTATATATGTCTCAGTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240606174006_xo3o ATCTGGCAGGTCTCATCTCCATTGGGTGGCAG(+AAAAAA)GATCCTGGGGAAGGAACCAACAGCTCCTCCG NGG 10 8 17 8 24 0 100 5 10
    240606174002_0qme GCCCGCGGAGGAGCTGTTGGTTCCTTCCCCAG(+AAAAAA)GATCCTGCCACCCAATGGAGATGAGACCTGC NGG 10 8 17 8 24 0 100 5 10
    240606174000_w3y5 GGGGCGGTGCGGGTGAGCGGCGCTGACCTTGG(+AAAAAA)GATCCCGCAGGCCTTCACACGTTCCAGCTGT NGG 10 8 17 8 24 0 100 5 10
    240606173958_97oy GAACAACAGCTGGAACGTGTGAAGGCCTGCGG(+AAAAAA)GATCCCAAGGTCAGCGCCGCTCACCCGCACC NGG 10 8 17 8 24 0 100 5 10
    240606173957_uz21 AGCCCAGAAGCAAAGCTGCTGAGGGGGACGTG(+AAAAAA)GTACCAGGTGGGGCTCAGCACTCATCCTCCC NGG 10 8 17 8 24 0 100 5 10
    240606173955_djln GCTCGGGGAGGATGAGTGCTGAGCCCCACCTG(+AAAAAA)GTACCACGTCCCCCTCAGCAGCTTTGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240606173954_kyjb GTGCAGTGCAGTGCAGTGCAGTGGGATAGATG(+AAAAAA)GTACCCCTCACCACCCCCTCGCCCTGTGAGG NGG 10 8 17 8 24 0 100 5 10