Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606174059_02ek AATCAAGACCCAGTTGTTGGAGAGATTCCTTG(+AAAAAA)GTACCGAGGACAAGTTCAGATGCTCTTCTGA NGG 10 8 17 8 24 0 100 5 10
    240606174058_zbzh TGAGCAATTAGAAAATAAGGCTGAATACAGAG(+AAAAAA)GTACCTTTAAAATTACAAAGAGACTTAACAT NGG 10 8 17 8 24 0 100 5 10
    240606174057_vkxn GCCTTATGTTAAGTCTCTTTGTAATTTTAAAG(+AAAAAA)GTACCTCTGTATTCAGCCTTATTTTCTAATT NGG 10 8 17 8 24 0 100 5 10
    240606174055_ibzl GCATCTGAAAGGCATTACCTAGCAGGAATGAG(+AAAAAA)GTACCCTGTGATCACTATTCCTGGTTATCAA NGG 10 8 17 8 24 0 100 5 10
    240606174054_x6k2 TCAAGTTGATAACCAGGAATAGTGATCACAGG(+AAAAAA)GTACCTCATTCCTGCTAGGTAATGCCTTTCA NGG 10 8 17 8 24 0 100 5 10
    240606174052_szto ATTGTAGTACAACTTTGAATCATCTCAGCAGG(+AAAAAA)GATCCTCGATTGTGAAACTGTCTTGATCATC NGG 10 8 17 8 24 0 100 5 10
    240606174051_ow8a ATTAAGATGATCAAGACAGTTTCACAATCGAG(+AAAAAA)GATCCCTGCTGAGATGATTCAAAGTTGTACT NGG 10 8 17 8 24 0 100 5 10
    240606174049_8tr4 CCTAAAGTTCCACAAGGGATGAAGAAGTCCTG(+AAAAAA)GATCCCCCTGTGGTACAGGTGAGAAAAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606174047_r8qo GTCTCCTCCTTTTCTCACCTGTACCACAGGGG(+AAAAAA)GATCCAGGACTTCTTCATCCCTTGTGGAACT NGG 10 8 17 8 24 0 100 5 10
    240606174044_2j3h CACCTCTTGACCTCTACACTTCAGGGGCGGGG(+AAAAAA)GTACCCCTCTGAGAATCTAGAAAGGAAGCAG NGG 10 8 17 8 24 0 100 5 10