Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170305_nt7g AGAGGATGACTTCTGCCAGGCTGTAGCTCTGG(+AAAAAA)GATCCTGTGCACTTTCTAGCTTCCACTGGAT NGG 10 8 17 8 24 0 100 5 10
    240606170303_7ltg GGGCTGTCTCTGACCCTATTACCTGCCTTTGG(+AAAAAA)GATCCATTCCCCCTCCTGCATCGTCCAACAT NGG 10 8 17 8 24 0 100 5 10
    240606170302_udnp TACTAATGTTGGACGATGCAGGAGGGGGAATG(+AAAAAA)GATCCCAAAGGCAGGTAATAGGGTCAGAGAC NGG 10 8 17 8 24 0 100 5 10
    240606170301_u3wi GCCCTCTTTCCTTGGCACATTCCATGTAGTTG(+AAAAAA)GTACCCTTGTGGGGACCCACAGCATCCATGG NGG 10 8 17 8 24 0 100 5 10
    240606170259_skzd GGTTTCCATGGATGCTGTGGGTCCCCACAAGG(+AAAAAA)GTACCAACTACATGGAATGTGCCAAGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606170258_fevz ATGCAGGTTAAGTGCCTAAGGTTTCCTGTTGG(+AAAAAA)GTACCACATGCTTCTCTAAAAGGCTTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240606170256_g1yg GACTCCCTTAAAGCCTTTTAGAGAAGCATGTG(+AAAAAA)GTACCCAACAGGAAACCTTAGGCACTTAACC NGG 10 8 17 8 24 0 100 5 10
    240606170255_a1jx CACTAACTACTGTGACAAGAAACTTAGCAAAG(+AAAAAA)GTACCTGTGTGTACAGTGAGGGCAGCTGGCT NGG 10 8 17 8 24 0 100 5 10
    240606170254_jn2c CTCATAGCCAGCTGCCCTCACTGTACACACAG(+AAAAAA)GTACCTTTGCTAAGTTTCTTGTCACAGTAGT NGG 10 8 17 8 24 0 100 5 10
    240606170253_uuqd AGTCGTCCCTCTGTCAGCTTGGAGCGTCTCAG(+AAAAAA)GTACCCAGGCAGTCTCAGACTTCTGGGCCTC NGG 10 8 17 8 24 0 100 5 10