Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170222_q8uo ACTCCAGGGGTGTCTGCTGCCCTTAACTCTAG(+AAAAAA)GTACCAAGCATGCACACATACATACATGCAA NGG 10 8 17 8 24 0 100 5 10
    240606170221_bomy CCTGCCCAGCCCCTCCTCCCACAGGAACACAG(+AAAAAA)GTACCACCTGCACCCACGACAGCTTCTTTTC NGG 10 8 17 8 24 0 100 5 10
    240606170219_55gc TGTGAGAAAAGAAGCTGTCGTGGGTGCAGGTG(+AAAAAA)GTACCTGTGTTCCTGTGGGAGGAGGGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240606170218_ngw8 GTGAGTGTGTGGCTTGCTGTCGTGTCCTCTGG(+AAAAAA)GATCCCAAGCCACGCGGGTCTTCCCTCTGTA NGG 10 8 17 8 24 0 100 5 10
    240606170216_rwb1 CCATCTACAGAGGGAAGACCCGCGTGGCTTGG(+AAAAAA)GATCCCAGAGGACACGACAGCAAGCCACACA NGG 10 8 17 8 24 0 100 5 10
    240606170215_glz0 GAAGCCAGTTTCCTAAACACCATGCAGAAGTG(+AAAAAA)GTACCCTGGGGCCTGGAAGCTCAAGCTTCCT NGG 10 8 17 8 24 0 100 5 10
    240606170212_04i4 CACCTAGGAAGCTTGAGCTTCCAGGCCCCAGG(+AAAAAA)GTACCACTTCTGCATGGTGTTTAGGAAACTG NGG 10 8 17 8 24 0 100 5 10
    240606170211_n2yt TTCTGGAGCCTGGTTACTCCTTAGAATTCAAG(+AAAAAA)GATCCAAGTGCAAGAGCTTCAGGCTTCAGCT NGG 10 8 17 8 24 0 100 5 10
    240606170209_td00 CTGACAGCTGAAGCCTGAAGCTCTTGCACTTG(+AAAAAA)GATCCTTGAATTCTAAGGAGTAACCAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240606170208_clhm GGGAGGGTCTGCCCCCATTTTGACAGGCCAAG(+AAAAAA)GATCCTGGGACCACTTGGGGTTGCAGTGTAT NGG 10 8 17 8 24 0 100 5 10