Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170208_clhm GGGAGGGTCTGCCCCCATTTTGACAGGCCAAG(+AAAAAA)GATCCTGGGACCACTTGGGGTTGCAGTGTAT NGG 10 8 17 8 24 0 100 5 10
    240606170205_grjn AGGTCATACACTGCAACCCCAAGTGGTCCCAG(+AAAAAA)GATCCTTGGCCTGTCAAAATGGGGGCAGACC NGG 10 8 17 8 24 0 100 5 10
    240606170204_sn7b GGTGTCATTGCTTGGAGGCTAATTATGGGATG(+AAAAAA)GATCCCTGGATATGGCAGTCTCTAGATGGAC NGG 10 8 17 8 24 0 100 5 10
    240606170201_0iep GGATGGTCCATCTAGAGACTGCCATATCCAGG(+AAAAAA)GATCCATCCCATAATTAGCCTCCAAGCAATG NGG 10 8 17 8 24 0 100 5 10
    240606170159_lx5d GGTGCACAGGCCTCGGAAGGCAGAGGCAGGGG(+AAAAAA)GATCCCTACCGGTTTAGAATCTGCATGGGCT NGG 10 8 17 8 24 0 100 5 10
    240606170158_wqii TATGCAGCCCATGCAGATTCTAAACCGGTAGG(+AAAAAA)GATCCCCCTGCCTCTGCCTTCCGAGGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240606170156_nulq TGTCTCGGTCCCGGCCGCTGCTCTCCAGCCAG(+AAAAAA)GTACCCTCCTCACTCCCTGCTCGCGCAGTTC NGG 10 8 17 8 24 0 100 5 10
    240606170154_m2a4 AGTGCGAACTGCGCGAGCAGGGAGTGAGGAGG(+AAAAAA)GTACCTGGCTGGAGAGCAGCGGCCGGGACCG NGG 10 8 17 8 24 0 100 5 10
    240606170152_w4d6 GGCGCCGGGGGCGGTGCCGGCTCCAATCACGG(+AAAAAA)GATCCGCCCAAGGGCCCCAACGCGGTAGGCG NGG 10 8 17 8 24 0 100 5 10
    240606170151_abck GGAAGCGCCTACCGCGTTGGGGCCCTTGGGCG(+AAAAAA)GATCCCGTGATTGGAGCCGGCACCGCCCCCG NGG 10 8 17 8 24 0 100 5 10