Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165828_bcw8 GCCGCGCCATATCTCGCGGCCAGTTCCTGCAG(+AAAAAA)GTACCGGCTCGCCCTGCCAGTCGCAAGCCAG NGG 10 8 17 8 24 0 100 5 10
    240606165826_eg4b CGGGGCTGGCTTGCGACTGGCAGGGCGAGCCG(+AAAAAA)GTACCTGCAGGAACTGGCCGCGAGATATGGC NGG 10 8 17 8 24 0 100 5 10
    240606165824_mmu5 AGACCCACCGAGGGGGAAGAGTGTGTCAAGGG(+AAAAAA)GTACCTAGGAGACCTGCTAAGCCGTCCCAGC NGG 10 8 17 8 24 0 100 5 10
    240606165822_rfn6 CTGTTGCTGGGACGGCTTAGCAGGTCTCCTAG(+AAAAAA)GTACCCCTTGACACACTCTTCCCCCTCGGTG NGG 10 8 17 8 24 0 100 5 10
    240606165820_8rik TTATAGTCCGTCATCCAGAGAAGTCAGGGCGG(+AAAAAA)GATCCTGGAGGCAAGGACTGAAGCAGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240606165818_bxg9 TCCACAGCCTCTGCTTCAGTCCTTGCCTCCAG(+AAAAAA)GATCCCGCCCTGACTTCTCTGGATGACGGAC NGG 10 8 17 8 24 0 100 5 10
    240606165816_dxy9 CGCATCTGTAGTACCAGAACTCAAGGCAGGAG(+AAAAAA)GATCCTTGCTAAGTTTGAAGCCAGCCTAGGC NGG 10 8 17 8 24 0 100 5 10
    240606165815_w186 AAGTAGCCTAGGCTGGCTTCAAACTTAGCAAG(+AAAAAA)GATCCTCCTGCCTTGAGTTCTGGTACTACAG NGG 10 8 17 8 24 0 100 5 10
    240606165813_xx43 GCCACTAGGCGAGTGGAGGAAGCTATAACCAG(+AAAAAA)GATCCTGGGGGAGAGGGGCTGCCTACGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606165810_y91e CCTTCCCTCCGTAGGCAGCCCCTCTCCCCCAG(+AAAAAA)GATCCTGGTTATAGCTTCCTCCACTCGCCTA NGG 10 8 17 8 24 0 100 5 10