Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165917_r052 GTCCACCTAATATAGCAGGTGGCCAGGACTGG(+AAAAAA)GATCCAGCTGCCTGGATCAGGTCAGGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240606165915_sp0m CTTCCTCAAGCCTGACCTGATCCAGGCAGCTG(+AAAAAA)GATCCCAGTCCTGGCCACCTGCTATATTAGG NGG 10 8 17 8 24 0 100 5 10
    240606165914_dlwr GTCAGCACTGTCCTTTGTGGAGATCTCACGAG(+AAAAAA)GATCCTCTGGGGTCTCCTGGAGTCATGATGT NGG 10 8 17 8 24 0 100 5 10
    240606165912_a3la ATGACACATCATGACTCCAGGAGACCCCAGAG(+AAAAAA)GATCCTCGTGAGATCTCCACAAAGGACAGTG NGG 10 8 17 8 24 0 100 5 10
    240606165909_1a39 TCCCTTCCTCCCTCCCTCCCTCCCTCCCCTGG(+AAAAAA)GTACCCTCTTACTGCCTGCCACCTTCTTGGC NGG 10 8 17 8 24 0 100 5 10
    240606165907_ipwk AGGTAGCCAAGAAGGTGGCAGGCAGTAAGAGG(+AAAAAA)GTACCCAGGGGAGGGAGGGAGGGAGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240606165906_3boo GTGCACATCAGTGATTTCTGTATATAAGGAGG(+AAAAAA)GTACCAAGTACCCTCTACAAGGTTCCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606165904_nw3r GGAGACCTGGGGAACCTTGTAGAGGGTACTTG(+AAAAAA)GTACCCTCCTTATATACAGAAATCACTGATG NGG 10 8 17 8 24 0 100 5 10
    240606165903_gynp GTCTAGACTTAGTATCTGGGTGTGCTGTCCAG(+AAAAAA)GATCCACAACTAAGCTTGTCTGCAGTTGAGA NGG 10 8 17 8 24 0 100 5 10
    240606165901_h1d4 CATGGTCTCAACTGCAGACAAGCTTAGTTGTG(+AAAAAA)GATCCTGGACAGCACACCCAGATACTAAGTC NGG 10 8 17 8 24 0 100 5 10