Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170012_p09z CAGTAGAACAGCACTGCTTGAGGCTCACATGG(+AAAAAA)GTACCAAATCCCTCTATTTCCTGGCTTACTG NGG 10 8 17 8 24 0 100 5 10
    240606170010_d4ym CAGGACAGTAAGCCAGGAAATAGAGGGATTTG(+AAAAAA)GTACCCATGTGAGCCTCAAGCAGTGCTGTTC NGG 10 8 17 8 24 0 100 5 10
    240606170009_isj9 CACCACCCCAGAGGTCAGCCTTTGGTGCATGG(+AAAAAA)GATCCAAGCCAGGACAAAGCACAAGCACAGA NGG 10 8 17 8 24 0 100 5 10
    240606170007_3xzg TCCAGTCTGTGCTTGTGCTTTGTCCTGGCTTG(+AAAAAA)GATCCCATGCACCAAAGGCTGACCTCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240606170005_3k3u CATCACCCTGTGCTACCAGCATCTGGTCCCAG(+AAAAAA)GATCCAGTCAGGCTAGGATTGTCAAAAGTTC NGG 10 8 17 8 24 0 100 5 10
    240606170004_w4af CTTGTGAACTTTTGACAATCCTAGCCTGACTG(+AAAAAA)GATCCTGGGACCAGATGCTGGTAGCACAGGG NGG 10 8 17 8 24 0 100 5 10
    240606170002_qybc GAGGGGGGTGTGAAAGCCCACAAACCTTCCTG(+AAAAAA)GATCCCAGCCTCCCTTCACTGGGGTACAGAC NGG 10 8 17 8 24 0 100 5 10
    240606170000_l9sj TGGGTGTCTGTACCCCAGTGAAGGGAGGCTGG(+AAAAAA)GATCCAGGAAGGTTTGTGGGCTTTCACACCC NGG 10 8 17 8 24 0 100 5 10
    240606165959_dnzk GCATGTTAATGCTCCCCCCCACCCCGGCTTTG(+AAAAAA)GTACCGGGGGGGGGGGCAGGAAACTGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240606165955_ye86 CCCCGTGCCTCAGTTTCCTGCCCCCCCCCCCG(+AAAAAA)GTACCAAAGCCGGGGTGGGGGGGAGCATTAA NGG 10 8 17 8 24 0 100 5 10