Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606170030_5x54 CTATGGGCCCTTTGGTGACATGTGGGGCTCTG(+AAAAAA)GATCCTTGGGTGTCCTCACTGTGGCCACTCT NGG 10 8 17 8 24 0 100 5 10
    240606170027_6hlo CAGGCAGAGTGGCCACAGTGAGGACACCCAAG(+AAAAAA)GATCCAGAGCCCCACATGTCACCAAAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606170025_1kq6 TGTTGCTTTCGGGACCCAAAGACTCTTCCGGG(+AAAAAA)GATCCAGCATCTTGCCTTCCATTCTCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606170024_45um CAGGCAGAAAGAGAATGGAAGGCAAGATGCTG(+AAAAAA)GATCCCCGGAAGAGTCTTTGGGTCCCGAAAG NGG 10 8 17 8 24 0 100 5 10
    240606170022_eu9n CACAGATGAGAATCTAACTTGGACCCCACCTG(+AAAAAA)GTACCCCACCTGGTACACTGCACATCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240606170020_2yym AAAGGACACTGATGTGCAGTGTACCAGGTGGG(+AAAAAA)GTACCAGGTGGGGTCCAAGTTAGATTCTCAT NGG 10 8 17 8 24 0 100 5 10
    240606170017_3kk3 TAGTGGGTCATGGGCTCAGGTCCTTATCCCAG(+AAAAAA)GATCCTCTAAGCCTTAGGTGTTGTGGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240606170016_a135 CAGACTCTTGCCACAACACCTAAGGCTTAGAG(+AAAAAA)GATCCTGGGATAAGGACCTGAGCCCATGACC NGG 10 8 17 8 24 0 100 5 10
    240606170015_3txu AAAAGGAGTCATTCCACAATGTCTGTATGAGG(+AAAAAA)GATCCAAACTCTGTCCTCTTTAATAAAGTCA NGG 10 8 17 8 24 0 100 5 10
    240606170013_xuw8 TATTCTGACTTTATTAAAGAGGACAGAGTTTG(+AAAAAA)GATCCCTCATACAGACATTGTGGAATGACTC NGG 10 8 17 8 24 0 100 5 10