Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165724_tt68 ACTGCCAACCTGGAACCAAGTGTGCAATACCG(+AAAAAA)GATCCTGTGGGGATGTCTCTCACTCAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240606165722_v0cb TGAGGTGGCTTGAGTGAGAGACATCCCCACAG(+AAAAAA)GATCCGGTATTGCACACTTGGTTCCAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240606165720_xpc4 CCCCAGCCAACTCAGGTGAGAGTGTGCTGCAG(+AAAAAA)GATCCTGGAGAGCAGGGTCAGGGGGGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240606165719_mgtx ACCCTGCCTGCCCCCCTGACCCTGCTCTCCAG(+AAAAAA)GATCCTGCAGCACACTCTCACCTGAGTTGGC NGG 10 8 17 8 24 0 100 5 10
    240606165717_0x9o ATGTCCCAACCAGCACCTTGGCCACATTCCTG(+AAAAAA)GATCCTATGCTTGTCCCAGAGGAGAAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240606165716_j9kk CTCTGCAGCCTTCTCCTCTGGGACAAGCATAG(+AAAAAA)GATCCAGGAATGTGGCCAAGGTGCTGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240606165714_n4rd AGAATCGCTTAAGCAGGGCTGTGGGCCTGCAG(+AAAAAA)GATCCCCAGGTCCCTTTGGACCGGAGGGGGA NGG 10 8 17 8 24 0 100 5 10
    240606165711_9eih CGTCTTCCCCCTCCGGTCCAAAGGGACCTGGG(+AAAAAA)GATCCTGCAGGCCCACAGCCCTGCTTAAGCG NGG 10 8 17 8 24 0 100 5 10
    240606165710_qhey TTGTGGGTCATGAATTCTCTGTACCCCTGCTG(+AAAAAA)GATCCTTCTAATTAAATTTGTTTTTCCTTCG NGG 10 8 17 8 24 0 100 5 10
    240606165709_cv6d GGTGACGAAGGAAAAACAAATTTAATTAGAAG(+AAAAAA)GATCCAGCAGGGGTACAGAGAATTCATGACC NGG 10 8 17 8 24 0 100 5 10