Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165615_kr7z TTATTTTTCTAAATAGTGTTCAGAAAACTCTG(+AAAAAA)GATCCAAAATGTGGCATTTTTCTGAGAATGA NGG 10 8 17 8 24 0 100 5 10
    240606165613_c3sr AATCTTCATTCTCAGAAAAATGCCACATTTTG(+AAAAAA)GATCCAGAGTTTTCTGAACACTATTTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606165612_nlyk ACTAAGCTGATCTGGCTATCCCAATGCATGTG(+AAAAAA)GTACCTGATAGATGGATATGGTAGAGATTAT NGG 10 8 17 8 24 0 100 5 10
    240606165611_61k6 GGGAAATAATCTCTACCATATCCATCTATCAG(+AAAAAA)GTACCACATGCATTGGGATAGCCAGATCAGC NGG 10 8 17 8 24 0 100 5 10
    240606165609_vcp9 TTATCATCTCTGACCTTCTGAAGCCAGGGGTG(+AAAAAA)GTACCCATCCCTAGCTCAGTCTAATTAAATC NGG 10 8 17 8 24 0 100 5 10
    240606165607_mg5f GGAAAGATTTAATTAGACTGAGCTAGGGATGG(+AAAAAA)GTACCACCCCTGGCTTCAGAAGGTCAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240606165606_q3uk TGTACTTTGGGCAGGTGATGAAATTTACACAG(+AAAAAA)GTACCTCAAGTCAAGACCTGCGCGTGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240606165605_qkyf CCAAATGCAGCACGCGCAGGTCTTGACTTGAG(+AAAAAA)GTACCTGTGTAAATTTCATCACCTGCCCAAA NGG 10 8 17 8 24 0 100 5 10
    240606165603_9fmp TCTAGTCCCTTCTCCACATCACCTAAGGGTGG(+AAAAAA)GTACCTCCATTGCGATGTTGTGGCATCTTAA NGG 10 8 17 8 24 0 100 5 10
    240606165602_2ayi AAAAGTTAAGATGCCACAACATCGCAATGGAG(+AAAAAA)GTACCCACCCTTAGGTGATGTGGAGAAGGGA NGG 10 8 17 8 24 0 100 5 10