Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165707_904g CTGTGAAGTCTATTGTGGCCACAGACTGCTGG(+AAAAAA)GTACCAAGTCTCAGGAGAACCCAGCCTAGAT NGG 10 8 17 8 24 0 100 5 10
    240606165706_32kf CCTAAATCTAGGCTGGGTTCTCCTGAGACTTG(+AAAAAA)GTACCCAGCAGTCTGTGGCCACAATAGACTT NGG 10 8 17 8 24 0 100 5 10
    240606165704_6z0b GATAATCTATCATTTGAATGGCAAGAGACCTG(+AAAAAA)GTACCCTCTATCTCGCTACTCTGGAGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240606165702_iogo CTAGGGGAGGCTCCAGAGTAGCGAGATAGAGG(+AAAAAA)GTACCAGGTCTCTTGCCATTCAAATGATAGA NGG 10 8 17 8 24 0 100 5 10
    240606165700_5wrz TCAACTCAGGCTCCCCATGACACTGGACCTGG(+AAAAAA)GTACCACAAGAAACCGGGTGAGGCCTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240606165658_3g3p TTCCTCTCCCAGGCCTCACCCGGTTTCTTGTG(+AAAAAA)GTACCCAGGTCCAGTGTCATGGGGAGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240606165657_s6iw GCCCACTCCTGCAGACACAGCAGATCCAGCTG(+AAAAAA)GTACCCTAGCTGCCGCCCAGGGCAACCGGCC NGG 10 8 17 8 24 0 100 5 10
    240606165656_w5n7 AGACTGGCCGGTTGCCCTGGGCGGCAGCTAGG(+AAAAAA)GTACCAGCTGGATCTGCTGTGTCTGCAGGAG NGG 10 8 17 8 24 0 100 5 10
    240606165653_yxu9 GGTTATCCGGGAAGTCCTGACAGGCCCCAGGG(+AAAAAA)GTACCAGCTGGGAGCAGGGTAACCTGGTGTC NGG 10 8 17 8 24 0 100 5 10
    240606165652_la0r GGAGGGACACCAGGTTACCCTGCTCCCAGCTG(+AAAAAA)GTACCCCTGGGGCCTGTCAGGACTTCCCGGA NGG 10 8 17 8 24 0 100 5 10