Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165600_im1k CAAGCAGCCATCTAACTGCTAGAGTATACTGG(+AAAAAA)GATCCTAACCTACCCTCTTGGGAAACGGAGG NGG 10 8 17 8 24 0 100 5 10
    240606165559_9k3f TTCCCCCTCCGTTTCCCAAGAGGGTAGGTTAG(+AAAAAA)GATCCCAGTATACTCTAGCAGTTAGATGGCT NGG 10 8 17 8 24 0 100 5 10
    240606165558_kkp4 GTCGCCACCCCCGCCTTTGGAATGTGGGAATG(+AAAAAA)GATCCATGAGGACACGCATGGTCACTCACTT NGG 10 8 17 8 24 0 100 5 10
    240606165556_og8b TCAGTAAGTGAGTGACCATGCGTGTCCTCATG(+AAAAAA)GATCCATTCCCACATTCCAAAGGCGGGGGTG NGG 10 8 17 8 24 0 100 5 10
    240606165555_9re5 TTCTAGTGTTCATCCCATTTGGATCCTTAAAG(+AAAAAA)GTACCCCATCCCAGGAAGAAGGTGCTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240606165552_i206 TGGGTGAGACAGCACCTTCTTCCTGGGATGGG(+AAAAAA)GTACCTTTAAGGATCCAAATGGGATGAACAC NGG 10 8 17 8 24 0 100 5 10
    240606165551_81qi CTTGTAGACATTTATCTTCAGGCGTCATCTTG(+AAAAAA)GTACCAGTGGATCCCAGAGGACACACCCAGG NGG 10 8 17 8 24 0 100 5 10
    240606165549_v4h7 TTGGTCCTGGGTGTGTCCTCTGGGATCCACTG(+AAAAAA)GTACCAAGATGACGCCTGAAGATAAATGTCT NGG 10 8 17 8 24 0 100 5 10
    240606165547_16og GTACATGGTGTTGAGGCACTGAGAGAACCAAG(+AAAAAA)GATCCACTCTCAGTTCCTACCCAGAAGGGTC NGG 10 8 17 8 24 0 100 5 10
    240606165546_ymx1 TTCTGGACCCTTCTGGGTAGGAACTGAGAGTG(+AAAAAA)GATCCTTGGTTCTCTCAGTGCCTCAACACCA NGG 10 8 17 8 24 0 100 5 10