Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165444_qqdm TTCATTGACCAACCAGTCTTAAGAATGAAGTG(+AAAAAA)GTACCCAGGCTCATTGAGAGATCCTGTCTCA NGG 10 8 17 8 24 0 100 5 10
    240606165441_vgzd TTTTTTGAGACAGGATCTCTCAATGAGCCTGG(+AAAAAA)GTACCACTTCATTCTTAAGACTGGTTGGTCA NGG 10 8 17 8 24 0 100 5 10
    240606165440_qdmf ATATGGCTATAAATATAAATTTTGTATTACTG(+AAAAAA)GTACCAAAAACACTTTTTTTTTTTTTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240606165439_4ymo CCCTGCTGGAAAAAAAAAAAAAAGTGTTTTTG(+AAAAAA)GTACCAGTAATACAAAATTTATATTTATAGC NGG 10 8 17 8 24 0 100 5 10
    240606165437_nyuv CTAGGTGTTCAGGGACCTCCAAAAGCTCCAAG(+AAAAAA)GATCCACCTCTTGCTACATCTTCAGTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240606165436_uz6k CTCTATTCCTACTGAAGATGTAGCAAGAGGTG(+AAAAAA)GATCCTTGGAGCTTTTGGAGGTCCCTGAACA NGG 10 8 17 8 24 0 100 5 10
    240606165434_g9yp TTGAGCGAGCGAGAGATTATTCTAATGTCCTG(+AAAAAA)GTACCCGGCTGGCTTCTGGGAGGACTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240606165432_kt86 TCGAAAAGCCAGTCCTCCCAGAAGCCAGCCGG(+AAAAAA)GTACCAGGACATTAGAATAATCTCTCGCTCG NGG 10 8 17 8 24 0 100 5 10
    240606165431_9xhk AAGCTTATTGGCCAAACCCTCAGGGCCTCTAG(+AAAAAA)GTACCTCATTAGCATGGAGAACTCTGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240606165430_vqnx ATCATCAGAACAGAGTTCTCCATGCTAATGAG(+AAAAAA)GTACCTAGAGGCCCTGAGGGTTTGGCCAATA NGG 10 8 17 8 24 0 100 5 10