Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606165412_5xie TTTCTTGATCTCAGAATAAATATTTCAAAGGG(+AAAAAA)GATCCAAAAATGCTGGACTTTTAAATATACT NGG 10 8 17 8 24 0 100 5 10
    240606165411_7uhg CTAAAAGTATATTTAAAAGTCCAGCATTTTTG(+AAAAAA)GATCCCCTTTGAAATATTTATTCTGAGATCA NGG 10 8 17 8 24 0 100 5 10
    240606165410_4eud ACAATGTTTGGAATGTTATATTGGGACAAATG(+AAAAAA)GTACCCAAGTGATCAGACATATAAAGTAGAC NGG 10 8 17 8 24 0 100 5 10
    240606165408_ghld CATTTGTCTACTTTATATGTCTGATCACTTGG(+AAAAAA)GTACCATTTGTCCCAATATAACATTCCAAAC NGG 10 8 17 8 24 0 100 5 10
    240606165406_7znr AACCTTTCTCAGACCCACAAGTCCCAAACCTG(+AAAAAA)GATCCCAGTTTTATCCCATAATTTCTTCAAT NGG 10 8 17 8 24 0 100 5 10
    240606165404_8dxe GTTGTATTGAAGAAATTATGGGATAAAACTGG(+AAAAAA)GATCCAGGTTTGGGACTTGTGGGTCTGAGAA NGG 10 8 17 8 24 0 100 5 10
    240606165403_xexx CTACGTTCATCCAGGCAGCAACAGAAGCTACG(+AAAAAA)GATCCTTAACATATCAATGAGCTCTGAACTT NGG 10 8 17 8 24 0 100 5 10
    240606165402_7hu6 TATTTAAGTTCAGAGCTCATTGATATGTTAAG(+AAAAAA)GATCCGTAGCTTCTGTTGCTGCCTGGATGAA NGG 10 8 17 8 24 0 100 5 10
    240606165401_ap61 GGTGGAGTGGATTGATTGGAGGCTGAGAAAAG(+AAAAAA)GTACCAAAGCACAGAGGTTGAAACAATAAAT NGG 10 8 17 8 24 0 100 5 10
    240606165359_vj3t CTAGAATTTATTGTTTCAACCTCTGTGCTTTG(+AAAAAA)GTACCTTTTCTCAGCCTCCAATCAATCCACT NGG 10 8 17 8 24 0 100 5 10