Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163910_q57e GCTGGGCAGGTCAGTGGCAGAGGTGGAACACG(+AAAAAA)GATCCCTTCCTGACCGCGTGGTAGGTAACTT NGG 10 8 17 8 24 0 100 5 10
    240606163908_7itl GTCGAAAGTTACCTACCACGCGGTCAGGAAGG(+AAAAAA)GATCCGTGTTCCACCTCTGCCACTGACCTGC NGG 10 8 17 8 24 0 100 5 10
    240606163907_bqlo AGGAGTTAGTTAAATGCTGAAGAGGCTCTCAG(+AAAAAA)GTACCCACATCCTGAACCGTGGGGGCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240606163905_pmj9 CCTGGGCTCAGCCCCCACGGTTCAGGATGTGG(+AAAAAA)GTACCTGAGAGCCTCTTCAGCATTTAACTAA NGG 10 8 17 8 24 0 100 5 10
    240606163904_qdls CAGGACAGGTTAAAAGGAAACAGCCAAGTGAG(+AAAAAA)GTACCTTAGCTGGCAGCAGCGCGAGGCGTGA NGG 10 8 17 8 24 0 100 5 10
    240606163903_y89v CCTGAACCGTGGGGGCTGAGCCCAGGTGGATG(+AAAAAA)GATCCAGTGCAGAGTTACCAACCTCCTCGAT NGG 10 8 17 8 24 0 100 5 10
    240606163901_oxv9 TGCCCATCGAGGAGGTTGGTAACTCTGCACTG(+AAAAAA)GATCCATCCACCTGGGCTCAGCCCCCACGGT NGG 10 8 17 8 24 0 100 5 10
    240606163900_gq5a GCACTTTAGAGTGGAAAGTTTCTAGTTCAAAG(+AAAAAA)GATCCTTTCCTTTCCTGCGAGTGCTTTCTCC NGG 10 8 17 8 24 0 100 5 10
    240606163859_0i38 TCGGGGGAGAAAGCACTCGCAGGAAAGGAAAG(+AAAAAA)GATCCTTTGAACTAGAAACTTTCCACTCTAA NGG 10 8 17 8 24 0 100 5 10
    240606163858_xs31 AACAAAAACCAGTCTACAATCAATGTGGTAAG(+AAAAAA)GTACCTGGAGAGAGCTTCTATGTTTTTGTTT NGG 10 8 17 8 24 0 100 5 10