Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163828_h3bu GCGACATCGGGACAGAACATGAGGTTTGGAGG(+AAAAAA)GATCCTCGCTGAAGCCCCGACAGCTATTCCA NGG 10 8 17 8 24 0 100 5 10
    240606163827_sx0y GCTCTGCAGAATCCAGCTTAATCCAGAAACAG(+AAAAAA)GTACCTAAAAGAGATGGCGTCGCTCTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240606163826_hx4l AACAAAAAGAAGAGCGACGCCATCTCTTTTAG(+AAAAAA)GTACCTGTTTCTGGATTAAGCTGGATTCTGC NGG 10 8 17 8 24 0 100 5 10
    240606163824_sktj TATTCCGGTTGAGGAGTAGACAACTGACTGGG(+AAAAAA)GATCCAAACCAATTTTTACATGGCCTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240606163822_agen TCACCCCTCAAGGCCATGTAAAAATTGGTTTG(+AAAAAA)GATCCCCAGTCAGTTGTCTACTCCTCAACCG NGG 10 8 17 8 24 0 100 5 10
    240606163821_bzs1 AGTCTTAGCTTGCTTCCTGGTATTCGTGGTAG(+AAAAAA)GATCCTGTCACAGGTCAATGTTAAGACTCAA NGG 10 8 17 8 24 0 100 5 10
    240606163820_1r0k AAATTTTGAGTCTTAACATTGACCTGTGACAG(+AAAAAA)GATCCTACCACGAATACCAGGAAGCAAGCTA NGG 10 8 17 8 24 0 100 5 10
    240606163818_nx3n ACAGGTCAACAACTTTCTTTCTTGGTTTCCTG(+AAAAAA)GTACCCTAGCCCCCCTCACACCCGTCTCACC NGG 10 8 17 8 24 0 100 5 10
    240606163816_qng5 GCAAAGGTGAGACGGGTGTGAGGGGGGCTAGG(+AAAAAA)GTACCAGGAAACCAAGAAAGAAAGTTGTTGA NGG 10 8 17 8 24 0 100 5 10
    240606163814_drkg CATCTGAGTTCATGCATCGAGCCTGGGCCTGG(+AAAAAA)GATCCAGTGAAGCTAAGCCCTCCTCCACCCA NGG 10 8 17 8 24 0 100 5 10