Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163727_hc6h TGAATGTGTTGTAGGCTGACCTCTGCATATGG(+AAAAAA)GTACCTCTGAATTGGTTTTTTTTTTCCCTTT NGG 10 8 17 8 24 0 100 5 10
    240606163725_wv6h TGTCAAAGCATCCCCGAACTTCCTGCCCTAGG(+AAAAAA)GATCCAGGAGGTAGCCATCCAGGAGGAGGTT NGG 10 8 17 8 24 0 100 5 10
    240606163723_c4k6 ATGACAACCTCCTCCTGGATGGCTACCTCCTG(+AAAAAA)GATCCCTAGGGCAGGAAGTTCGGGGATGCTT NGG 10 8 17 8 24 0 100 5 10
    240606163721_zudm AGTTTAATTGGCTAAGCCCTTCAGCCCTTTAG(+AAAAAA)GTACCTCATTAAAATGGAGATCTGTCTTGAG NGG 10 8 17 8 24 0 100 5 10
    240606163720_g4e0 AGAGTCTCAAGACAGATCTCCATTTTAATGAG(+AAAAAA)GTACCTAAAGGGCTGAAGGGCTTAGCCAATT NGG 10 8 17 8 24 0 100 5 10
    240606163719_s8s7 GGGATGGATGAAGTCGGTGCTGGCCATGCCTG(+AAAAAA)GTACCATGATACTGGTTCTGGGCCAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240606163718_rq5o AGCCACCTCTCTGGCCCAGAACCAGTATCATG(+AAAAAA)GTACCAGGCATGGCCAGCACCGACTTCATCC NGG 10 8 17 8 24 0 100 5 10
    240606163716_d8ot GGCTCTCACATCTATGAGATGGGGCTAAAATG(+AAAAAA)GTACCTGTCCCTGTAGACTAACGGTATGAGG NGG 10 8 17 8 24 0 100 5 10
    240606163715_6mh5 TTAATCCTCATACCGTTAGTCTACAGGGACAG(+AAAAAA)GTACCATTTTAGCCCCATCTCATAGATGTGA NGG 10 8 17 8 24 0 100 5 10
    240606163714_0cfz CCTGAAAGACTACTTGCTCTCTGCTATAGAAG(+AAAAAA)GATCCAGCACCCCTGTTCCCCACTTATAACT NGG 10 8 17 8 24 0 100 5 10