Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163646_hwh2 CCCTGGCTGCTTAGCTCAAATGGAGCCGTCTG(+AAAAAA)GATCCCTCCTGGGCATTGCTGCAGGGCACTC NGG 10 8 17 8 24 0 100 5 10
    240606163644_6ni0 TAAAGTTCAGACCTGGGGTGGGAGAGAAGAGG(+AAAAAA)GTACCGGAATGATTATTAATGTACAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240606163642_wjsg AGAATTGCATTTGTACATTAATAATCATTCCG(+AAAAAA)GTACCCTCTTCTCTCCCACCCCAGGTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240606163640_oxru TGGAGATCAGTCAACAAATTTGTAAATCCTTG(+AAAAAA)GATCCAAGTTTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606163639_501d CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+AAAAAA)GATCCAAGGATTTACAAATTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240606163638_nono TTTGTGTGTTTTGGATCTCATGGCAGTAATAG(+AAAAAA)GTACCCGAGGTGTTAACTACCGTTTCTGTAA NGG 10 8 17 8 24 0 100 5 10
    240606163636_f9yr TGCTGTTACAGAAACGGTAGTTAACACCTCGG(+AAAAAA)GTACCTATTACTGCCATGAGATCCAAAACAC NGG 10 8 17 8 24 0 100 5 10
    240606163634_wwic TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+AAAAAA)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606163633_t51y CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+AAAAAA)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240606163631_5ot5 TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+AAAAAA)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10