Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163614_zlyv CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+AAAAAA)GATCCAAGGATTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240606163613_teac TGGAGATCAGTCAACAAAATTGTAAATCCTTG(+AAAAAA)GATCCAAGTTTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606163611_g9y1 CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+AAAAAA)GATCCAAGGATTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240606163610_92uk CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+AAAAAA)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240606163608_7i3n TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+AAAAAA)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606163607_rz1q TGGAGATCAGTCAACAAAATTGTAAATCCTTG(+AAAAAA)GATCCAAGTTTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606163606_bcri CAAGTCAAAGCAGAACACATGTTGTAAACTTG(+AAAAAA)GATCCAAGGATTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240606163604_wpg9 CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+AAAAAA)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10
    240606163602_pyd4 TGGAGATCAGTCAACAAAATTGTAAAACCTTG(+AAAAAA)GATCCAAGTCTACAACATGTGTTCTGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240606163601_aexp CAAGTCAAAGCAGAACACATGTTGTAGACTTG(+AAAAAA)GATCCAAGGTTTTACAATTTTGTTGACTGAT NGG 10 8 17 8 24 0 100 5 10