Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606163713_dx5k CTGTAAGTTATAAGTGGGGAACAGGGGTGCTG(+AAAAAA)GATCCTTCTATAGCAGAGAGCAAGTAGTCTT NGG 10 8 17 8 24 0 100 5 10
    240606163712_s67r CACTGTCTCTCCCCCTCCCCCCAGGTAAATTG(+AAAAAA)GATCCTGCTCTGTGGGGGGCAGGGGAGTAAT NGG 10 8 17 8 24 0 100 5 10
    240606163711_8l2h CTCTTATTACTCCCCTGCCCCCCACAGAGCAG(+AAAAAA)GATCCAATTTACCTGGGGGGAGGGGGAGAGA NGG 10 8 17 8 24 0 100 5 10
    240606163710_jkzl GTCAGCTGCAGGGGGATCTGGGTAAGGAAAAG(+AAAAAA)GTACCGCTCTCCACAATCTCCTGTCTTTAAG NGG 10 8 17 8 24 0 100 5 10
    240606163709_hldl TCCAACTTAAAGACAGGAGATTGTGGAGAGCG(+AAAAAA)GTACCTTTTCCTTACCCAGATCCCCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240606163707_l09a TTTGTTTTGTTTTTTTTAACCCTCAAATTAGG(+AAAAAA)GTACCCTAATCAAGGCAAAAAACTTAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606163705_n8o7 AGCCATTTCTTAAGTTTTTTGCCTTGATTAGG(+AAAAAA)GTACCCTAATTTGAGGGTTAAAAAAAACAAA NGG 10 8 17 8 24 0 100 5 10
    240606163704_tcx3 GAGAATTTTATATAAAAGTAACAGAAATAAAG(+AAAAAA)GTACCAAAAAAGAAAAAAAGGAAAAAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240606163703_azmx TTGTTTTTCTTTTTTCCTTTTTTTCTTTTTTG(+AAAAAA)GTACCTTTATTTCTGTTACTTTTATATAAAA NGG 10 8 17 8 24 0 100 5 10
    240606163702_n6d5 TTTCAAGTGTCATTGCAATTTATTACTATTAG(+AAAAAA)GTACCAAGTGGGTGGGAATAATATACAGCAT NGG 10 8 17 8 24 0 100 5 10