Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162646_nhv4 AATTCACACCCTTGAAGAAAAGGTTATTACAG(+AAAAAA)GATCCAAAAGCAGTCCAGAAGGAACTGCAAG NGG 10 8 17 8 24 0 100 5 10
    240606162645_w4lr AGAACCTTGCAGTTCCTTCTGGACTGCTTTTG(+AAAAAA)GATCCTGTAATAACCTTTTCTTCAAGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240606162644_eu89 CAAGAGTTTAAGCCCTGCCCCATCTCTACTTG(+AAAAAA)GTACCCAATGACACTAACAAGGAAGCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240606162643_wkpj AGATAAAACAGCTTCCTTGTTAGTGTCATTGG(+AAAAAA)GTACCAAGTAGAGATGGGGCAGGGCTTAAAC NGG 10 8 17 8 24 0 100 5 10
    240606162642_g6bj CATCCACAGTTATGTATAAACCCACTGCAGTG(+AAAAAA)GATCCCAGGACACAAACCCAGAAGGAATTTC NGG 10 8 17 8 24 0 100 5 10
    240606162639_tx42 CCTGAGAAATTCCTTCTGGGTTTGTGTCCTGG(+AAAAAA)GATCCACTGCAGTGGGTTTATACATAACTGT NGG 10 8 17 8 24 0 100 5 10
    240606162638_1k7w CTGGTCCAGGGAAGGAACAGGTGTTAGGATGG(+AAAAAA)GTACCCAAGCTACAGATCTGATGAGGGTAAC NGG 10 8 17 8 24 0 100 5 10
    240606162636_y08h ATGGGGTTACCCTCATCAGATCTGTAGCTTGG(+AAAAAA)GTACCCATCCTAACACCTGTTCCTTCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240606162635_iffy CACGGCAAGAGCAAGGCTGCAAACTGTCTCTG(+AAAAAA)GTACCACGGAATGTGCTTTCCAACACACATG NGG 10 8 17 8 24 0 100 5 10
    240606162633_suoq CAGCCCATGTGTGTTGGAAAGCACATTCCGTG(+AAAAAA)GTACCAGAGACAGTTTGCAGCCTTGCTCTTG NGG 10 8 17 8 24 0 100 5 10