Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162736_m5o7 AAGGTACTACCGGCTATAGCCACAGGGATCCG(+AAAAAA)GTACCATTGAGATCCCTTCATAAAATATGGA NGG 10 8 17 8 24 0 100 5 10
    240606162734_l5u0 CTTGCTCCATATTTTATGAAGGGATCTCAATG(+AAAAAA)GTACCGGATCCCTGTGGCTATAGCCGGTAGT NGG 10 8 17 8 24 0 100 5 10
    240606162732_xurr TGCTCCCGCCTATCTTGCTCCTTAACACTGGG(+AAAAAA)GATCCGATAGAGAAATGACACAAGGTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240606162731_x0gh ACACACTGAAACCTTGTGTCATTTCTCTATCG(+AAAAAA)GATCCCCAGTGTTAAGGAGCAAGATAGGCGG NGG 10 8 17 8 24 0 100 5 10
    240606162729_eaas GCTCCTAAGGTACTACCGGCTATAGCCACAGG(+AAAAAA)GATCCGGTACCATTGAGATCCCTTCATAAAA NGG 10 8 17 8 24 0 100 5 10
    240606162728_x668 CCATATTTTATGAAGGGATCTCAATGGTACCG(+AAAAAA)GATCCCTGTGGCTATAGCCGGTAGTACCTTA NGG 10 8 17 8 24 0 100 5 10
    240606162726_87bs GGAGCCACAAGGAGTGGGCTTCATGCATTATG(+AAAAAA)GATCCCGACCCCCGCCACTGTGGGCTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240606162725_h6sv TCCTGGAGCAAGCCCACAGTGGCGGGGGTCGG(+AAAAAA)GATCCATAATGCATGAAGCCCACTCCTTGTG NGG 10 8 17 8 24 0 100 5 10
    240606162722_6tbu CCAGCAAGAAGGGGGAGCAGGGGACGAGGGGG(+AAAAAA)GTACCTATCCTTCCTGAACGGACAATGGGGG NGG 10 8 17 8 24 0 100 5 10
    240606162721_uzv6 CATCACCCCCATTGTCCGTTCAGGAAGGATAG(+AAAAAA)GTACCCCCCTCGTCCCCTGCTCCCCCTTCTT NGG 10 8 17 8 24 0 100 5 10