Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162837_1k73 AAAAATGGCAACAGAATTCAAATCATGACTTG(+AAAAAA)GATCCTCAATGATTCAAGTACCATGGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240606162835_npvi GCTTTCAATCATCTGTAACTCCAGTTCCAATG(+AAAAAA)GATCCAACACCTTATAGCCTCTGGTACACAT NGG 10 8 17 8 24 0 100 5 10
    240606162834_i6p0 TATGCATGTGTACCAGAGGCTATAAGGTGTTG(+AAAAAA)GATCCATTGGAACTGGAGTTACAGATGATTG NGG 10 8 17 8 24 0 100 5 10
    240606162832_iqwm CATACAGAGGGATACCACTGCTGTTGGGTCTG(+AAAAAA)GTACCAGACACTCAGGCCCTTCATCTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240606162831_8ai5 ACGGAGAAGAAGATGAAGGGCCTGAGTGTCTG(+AAAAAA)GTACCAGACCCAACAGCAGTGGTATCCCTCT NGG 10 8 17 8 24 0 100 5 10
    240606162830_g3wo AAAATCCATGCTGCAGGGTAACCCTGTAAGTG(+AAAAAA)GATCCACTGTGGAAACATTTTGTACATCACA NGG 10 8 17 8 24 0 100 5 10
    240606162829_b41h GATACTGTGATGTACAAAATGTTTCCACAGTG(+AAAAAA)GATCCACTTACAGGGTTACCCTGCAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240606162827_4ol0 GACATAAACTGGATATTGTTTCAATAGACAGG(+AAAAAA)GATCCCATTCCCCAAACCACCTGGTTGCTAA NGG 10 8 17 8 24 0 100 5 10
    240606162825_ahqb AATAGTTAGCAACCAGGTGGTTTGGGGAATGG(+AAAAAA)GATCCCTGTCTATTGAAACAATATCCAGTTT NGG 10 8 17 8 24 0 100 5 10
    240606162823_lhdg ACTCCCCAGAACATGCCACATCCCTTAATGGG(+AAAAAA)GATCCCAAATGAGACCTGAGGTGAAAGACAT NGG 10 8 17 8 24 0 100 5 10