Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162934_h5t7 TTCCTCTCAAGTGCTGGGATTAATGCAATGGG(+AAAAAA)GTACCAGGACTGGCAATAAAATTACCTACTT NGG 10 8 17 8 24 0 100 5 10
    240606162932_redy CTTAGAAGTAGGTAATTTTATTGCCAGTCCTG(+AAAAAA)GTACCCCATTGCATTAATCCCAGCACTTGAG NGG 10 8 17 8 24 0 100 5 10
    240606162931_jtz0 TGGGAAGACCAGCCCCCACCCAGAGAATATTG(+AAAAAA)GATCCTTGGATAAAAATCACAGACACAATGC NGG 10 8 17 8 24 0 100 5 10
    240606162929_ughf ATTGAGCATTGTGTCTGTGATTTTTATCCAAG(+AAAAAA)GATCCAATATTCTCTGGGTGGGGGCTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240606162927_e59y GGTGTCAGCATTTGGAGGCTGATTATGGGATG(+AAAAAA)GATCCCCGGATATGGCAGTCTCTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240606162925_s5nv GGATGGACCATCTAGAGACTGCCATATCCGGG(+AAAAAA)GATCCATCCCATAATCAGCCTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240606162923_dxo0 TTGTGGATATATGCCCAGGAGAGGTATTGCGG(+AAAAAA)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606162921_ic2y TCCTCAGAAAATTGGACATAGTACTACCGGAG(+AAAAAA)GATCCCGCAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10
    240606162919_ibu0 AGTTGTTCCAGCTGGACTTCTGTATAAGCTGG(+AAAAAA)GATCCTGAAGAAGTAGGTTCCAACAGATGTG NGG 10 8 17 8 24 0 100 5 10
    240606162918_txjo GCCAGCACATCTGTTGGAACCTACTTCTTCAG(+AAAAAA)GATCCCAGCTTATACAGAAGTCCAGCTGGAA NGG 10 8 17 8 24 0 100 5 10