Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162902_2jey TGTGTGTGAAACCAGAGATGAAAGCATGATTG(+AAAAAA)GATCCTTTATACTTGGTAAACAAACCTCATG NGG 10 8 17 8 24 0 100 5 10
    240606162901_wctk ATAGACATGAGGTTTGTTTACCAAGTATAAAG(+AAAAAA)GATCCAATCATGCTTTCATCTCTGGTTTCAC NGG 10 8 17 8 24 0 100 5 10
    240606162900_ncpv ATCCCTTCTACTAAGTGCATACATGTAGTTTG(+AAAAAA)GATCCATATTTCAATTCACTGTCAAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240606162859_tofd GTCATAGAAACTTGACAGTGAATTGAAATATG(+AAAAAA)GATCCAAACTACATGTATGCACTTAGTAGAA NGG 10 8 17 8 24 0 100 5 10
    240606162857_4ql5 TGTTTAATGTGAACTGATATATTTAAAACAAG(+AAAAAA)GATCCAGGGTAGACAGGGAGAATGTTTTAGT NGG 10 8 17 8 24 0 100 5 10
    240606162855_jd0m GTTCTACTAAAACATTCTCCCTGTCTACCCTG(+AAAAAA)GATCCTTGTTTTAAATATATCAGTTCACATT NGG 10 8 17 8 24 0 100 5 10
    240606162854_dwjl AAAAGGATATACCTTCTTCTCAGCACTTCATG(+AAAAAA)GTACCTCCTCCTAAATTGACCATATACCCAG NGG 10 8 17 8 24 0 100 5 10
    240606162853_efwn TGTGACTGGGTATATGGTCAATTTAGGAGGAG(+AAAAAA)GTACCATGAAGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240606162851_hobm ATGAAATGGACAACTTCCTAGACAGATACCAG(+AAAAAA)GTACCAAAGTTAAGTCAGAATCAGATTAATG NGG 10 8 17 8 24 0 100 5 10
    240606162850_l6jl TAGATCATTAATCTGATTCTGACTTAACTTTG(+AAAAAA)GTACCTGGTATCTGTCTAGGAAGTTGTCCAT NGG 10 8 17 8 24 0 100 5 10