Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162850_l6jl TAGATCATTAATCTGATTCTGACTTAACTTTG(+AAAAAA)GTACCTGGTATCTGTCTAGGAAGTTGTCCAT NGG 10 8 17 8 24 0 100 5 10
    240606162848_e2rm GGATAAAGGAACTCCGCCGGACTAGAGGCTGG(+AAAAAA)GATCCATTCCGGTTGGGGCCAGCCCCGCCAT NGG 10 8 17 8 24 0 100 5 10
    240606162847_0r7d GGAAGATGGCGGGGCTGGCCCCAACCGGAATG(+AAAAAA)GATCCCAGCCTCTAGTCCGGCGGAGTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240606162846_e2wj TTCTTTCTTTGTGTTGGTTGGACTTTAAGAGG(+AAAAAA)GTACCAAGCTGGAGTGGATGCATAAGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240606162844_4e5j TTTCTACTTCCTTATGCATCCACTCCAGCTTG(+AAAAAA)GTACCCTCTTAAAGTCCAACCAACACAAAGA NGG 10 8 17 8 24 0 100 5 10
    240606162843_hits ACAGAGTGTACAACTCAGGGTAGCAACTGAAG(+AAAAAA)GTACCTGGTCAAAATGACACCTACAGATGAG NGG 10 8 17 8 24 0 100 5 10
    240606162841_6qgw AAAATCTCATCTGTAGGTGTCATTTTGACCAG(+AAAAAA)GTACCTTCAGTTGCTACCCTGAGTTGTACAC NGG 10 8 17 8 24 0 100 5 10
    240606162840_ijzw TATAGTAAAGTCAATGATGCTACATTCACTAG(+AAAAAA)GATCCAGATACTGATTGTCTGTGAAGGACTA NGG 10 8 17 8 24 0 100 5 10
    240606162839_gy5v CTCGTTAGTCCTTCACAGACAATCAGTATCTG(+AAAAAA)GATCCTAGTGAATGTAGCATCATTGACTTTA NGG 10 8 17 8 24 0 100 5 10
    240606162838_zbz1 TTGAGTCAAACCATGGTACTTGAATCATTGAG(+AAAAAA)GATCCAAGTCATGATTTGAATTCTGTTGCCA NGG 10 8 17 8 24 0 100 5 10