Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606162822_0z22 CTTCAATGTCTTTCACCTCAGGTCTCATTTGG(+AAAAAA)GATCCCCATTAAGGGATGTGGCATGTTCTGG NGG 10 8 17 8 24 0 100 5 10
    240606162820_36yb ACTTCCCCAATTCAGTATCCCCTGACAAGTGG(+AAAAAA)GATCCTGCACTCAAGTCCCTTAAATATATAC NGG 10 8 17 8 24 0 100 5 10
    240606162819_1g2e TCATAGTATATATTTAAGGGACTTGAGTGCAG(+AAAAAA)GATCCCACTTGTCAGGGGATACTGAATTGGG NGG 10 8 17 8 24 0 100 5 10
    240606162817_mzjx GCCTCCCCAACTCAGTAGCCCCTGACAAGTGG(+AAAAAA)GATCCTGCACTCAAGTCCCTTAAATCCATAC NGG 10 8 17 8 24 0 100 5 10
    240606162816_iiqd TCATAGTATGGATTTAAGGGACTTGAGTGCAG(+AAAAAA)GATCCCACTTGTCAGGGGCTACTGAGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240606162814_sb3u GATCCCTCTGCACCACGTGTCTTAGAATTAAG(+AAAAAA)GATCCAAAAGAAGAAAAAAAGTTACCATGGT NGG 10 8 17 8 24 0 100 5 10
    240606162813_g5mx TCAACACCATGGTAACTTTTTTTCTTCTTTTG(+AAAAAA)GATCCTTAATTCTAAGACACGTGGTGCAGAG NGG 10 8 17 8 24 0 100 5 10
    240606162811_ujzn TCAACCTTCTGAGGCATGTGCTACCATGCCTG(+AAAAAA)GATCCCAAGTTTTTCCTTAAGTGGTCTTGAT NGG 10 8 17 8 24 0 100 5 10
    240606162809_kmv2 AGTCAATCAAGACCACTTAAGGAAAAACTTGG(+AAAAAA)GATCCAGGCATGGTAGCACATGCCTCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240606162807_zzji GGAACTTTTCAGAAGATGCTTAGTGAAAGGGG(+AAAAAA)GATCCGTTCAAATAGATGTCTGGTCCCTGCA NGG 10 8 17 8 24 0 100 5 10